Department of Colorectal Surgery, The Sixth Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, PR China.
Oncol Rep. 2013 Aug;30(2):659-66. doi: 10.3892/or.2013.2533. Epub 2013 Jun 11.
The aim of the present study was to investigate the expression dynamics of CCL21 and its prognostic significance in human stage III/IV colorectal cancer (CRC). CCL21 expression dynamics were detected with western blotting. The expression of CCL21 in CRC tissue microarrays was examined by immunohistochemistry. The optimal cut-point of CCL21 expression was assessed by the X-tile program. The prognostic significance was analyzed using both Kaplan-Meier curves and Cox regression analysis. Western blot analysis demonstrated that CCL21 expression was comparable in the CRC and normal colorectal tissues. According to the X-tile program, the cut-point for high expression of CCL21 in CRC was determined when the CCL21 expression index was >56.1. Overexpression of CCL21 was significantly correlated with larger tumor diameter, more mucinous carcinoma or signet ring cell carcinoma and poor tumor differentiation. Patients with high expression of CCL21 had a higher overall survival rate in comparison to patients with low expression. In the multivariate Cox regression analysis, CCL21 expression was found to be an independent prognostic biomarker for CRC. ROC curves showed that CCL21 expression could improve the prognostic capability of TNM stage in stage III/IV CRC patients. High expression of CCL21 is an independent and useful biomarker for predicting longer survival of stage III/IV CRC patients.
本研究旨在探讨趋化因子配体 21（CCL21）在人 III/IV 期结直肠癌（CRC）中的表达动态及其预后意义。采用 Western blot 检测 CCL21 的表达动态。采用免疫组织化学法检测 CRC 组织微阵列中 CCL21 的表达。采用 X-tile 程序评估 CCL21 表达的最佳截断值。采用 Kaplan-Meier 曲线和 Cox 回归分析评估预后意义。Western blot 分析表明，CCL21 在 CRC 和正常结直肠组织中的表达相当。根据 X-tile 程序，当 CCL21 表达指数>56.1 时，确定 CRC 中 CCL21 高表达的截断值。CCL21 的过表达与肿瘤直径较大、更多黏液性癌或印戒细胞癌以及肿瘤分化不良显著相关。与低表达患者相比，高表达 CCL21 的患者总生存率更高。在多变量 Cox 回归分析中，CCL21 表达被发现是 CRC 的独立预后生物标志物。ROC 曲线表明，CCL21 表达可提高 III/IV 期 CRC 患者 TNM 分期的预后能力。CCL21 高表达是预测 III/IV 期 CRC 患者生存时间延长的独立且有用的生物标志物。
