Department of Medicine, Faculty of Medicine, Naresuan University, Phitsanulok, Thailand.
Biol Res. 2013;46(1):39-45. doi: 10.4067/S0716-97602013000100006.
Carotenoids are efficient antioxidants that are of great importance for human health. Lutein and zeaxanthin are carotinoids present in high concentrations in the human retina which are involved in the photoprotection of the human eye. Lutein may also protect the skin from ultraviolet (UV)-induced damage. The present study investigated the protective effect of lutein extracted from yellow silk cocoons of Bombyx mori on human keratinocytes against UVB irradiation. A human keratinocyte cell line and primary human keratinocytes were used to investigate the UVB protection effects of silk lutein and plant lutein. Silk lutein showed no cytotoxicity to keratinocytes. Treatment with silk lutein prior to UVB irradiation enhanced cell viability and cell proliferation, and reduced cell apoptosis. The protective effects of silk lutein may be superior to those of plant lutein. Silk lutein may have a benefit for protection of keratinocytes against UVB-irradiation.
类胡萝卜素是一种高效的抗氧化剂，对人类健康具有重要意义。叶黄素和玉米黄质是人类视网膜中高浓度存在的类胡萝卜素，它们参与了人类眼睛的光保护。叶黄素还可以保护皮肤免受紫外线（UV）诱导的损伤。本研究调查了从家蚕黄茧中提取的叶黄素对人角质形成细胞抵抗 UVB 照射的保护作用。使用人角质形成细胞系和原代人角质形成细胞来研究丝质叶黄素和植物叶黄素对 UVB 的保护作用。丝质叶黄素对角质形成细胞没有细胞毒性。在 UVB 照射前用丝质叶黄素处理可提高细胞活力和增殖，并减少细胞凋亡。丝质叶黄素的保护作用可能优于植物叶黄素。丝质叶黄素可能对保护角质形成细胞免受 UVB 照射有益。
