Psotova Jitka, Svobodova Alena, Kolarova Hana, Walterova Daniela
Institute of Medical Chemistry and Biochemistry, Faculty of Medicine, Palacky University, Hnevotínská 3, 775 15 Olomouc, Czech Republic.
J Photochem Photobiol B. 2006 Sep 1;84(3):167-74. doi: 10.1016/j.jphotobiol.2006.02.012. Epub 2006 Apr 21.
UVA radiation provokes the generation of reactive oxygen species (ROS), which induce oxidative stress in the exposed cells leading to extensive cellular damage and cell death either by apoptosis or necrosis. One approach to protecting human skin against the harmful effects of UV radiation is by using herbal compounds as photoprotectants. This study evaluated the protective effects of Prunella vulgaris L. (Labiatae) and its main phenolic acid component, rosmarinic acid (RA), against UVA-induced changes in a human keratinocyte cell line (HaCaT). Human keratinocytes exposed to UVA (10-30 J/cm(2)) were treated with an extract of P. vulgaris (1-75 mg/l) or RA (0.9-18 mg/l) for 4h. P. vulgaris and RA exhibited ability to reduce the UVA-caused decrease in a cell viability monitored by neutral red retention and by LDH release into medium. The P. vulgaris extract and RA significantly suppressed UVA-induced ROS production, which manifests as a decrease in intracellular lipid peroxidation, elevation of ATP and reduced glutathione. Post-treatment with P. vulgaris extract and RA also significantly reduced DNA damage. In addition, UVA-induced activation of caspase-3 was inhibited by treatment with P. vulgaris and RA. The P. vulgaris extract and RA demonstrated a concentration-dependent photoprotection (maximum at 25-50 mg/l and 9 mg/l, respectively). These results suggest that P. vulgaris and RA, used in skin care cosmetics, may offer protection against UVA-induced oxidative stress and may be beneficial as a supplement in photoprotective dermatological preparations.
紫外线A辐射会引发活性氧（ROS）的产生，活性氧会在暴露的细胞中诱导氧化应激，进而导致广泛的细胞损伤以及细胞通过凋亡或坏死途径死亡。保护人类皮肤免受紫外线辐射有害影响的一种方法是使用草药化合物作为光保护剂。本研究评估了夏枯草（唇形科）及其主要酚酸成分迷迭香酸（RA）对人角质形成细胞系（HaCaT）中紫外线A诱导变化的保护作用。将暴露于紫外线A（10 - 30 J/cm²）的人角质形成细胞用夏枯草提取物（1 - 75 mg/l）或迷迭香酸（0.9 - 18 mg/l）处理4小时。夏枯草和迷迭香酸表现出能够减少紫外线A导致的细胞活力下降，这通过中性红保留和乳酸脱氢酶释放到培养基中进行监测。夏枯草提取物和迷迭香酸显著抑制了紫外线A诱导的ROS产生，表现为细胞内脂质过氧化减少、ATP升高和谷胱甘肽还原。用夏枯草提取物和迷迭香酸进行后处理也显著减少了DNA损伤。此外，夏枯草和迷迭香酸处理可抑制紫外线A诱导的半胱天冬酶 - 3激活。夏枯草提取物和迷迭香酸表现出浓度依赖性的光保护作用（分别在25 - 50 mg/l和9 mg/l时达到最大值）。这些结果表明，用于护肤品中的夏枯草和迷迭香酸可能提供针对紫外线A诱导的氧化应激的保护，并且作为光保护性皮肤病制剂中的补充剂可能是有益的。
