From the 1Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong SAR; 2Nestlé Research Center Beijing, Haidian District, Beijing, China; 3Shenzhen Research Institute of The Hong Kong Polytechnic University, State Key Laboratory of Chinese Medicine and Molecular Pharmacology (Incubation), Shenzhen, China; and 4Nestlé Research Center, Lausanne, Switzerland.
Menopause. 2014 Mar;21(3):286-94. doi: 10.1097/GME.0b013e3182966fd3.
The aim of this study was to evaluate the efficacy of Fructus Ligustri Lucidi (FLL) and Puerariae radix (PR) combination treatment in bone and mineral metabolism in ovariectomized (OVX) rats in our search for an alternative regimen for the management of postmenopausal osteoporosis.
Six-month-old OVX rats were used as postmenopausal osteoporotic models, and PR water extract (PR) and FLL water extract (WE) were added to commercial diets individually or in combination and administered to OVX rats for 12 weeks. Bone properties, calcium and phosphorus absorption, and bone biochemical markers were measured to investigate the potential interactions between the actions of PR and the actions of WE on bone and mineral metabolism in OVX rats.
Long-term treatment with PR did not significantly improve bone properties but greatly ameliorated the secondary hyperparathyroidism induced by ovariectomy in the animals. WE significantly enhanced the intestinal calcium absorption rate and decreased the enlarged trabecular bone surface at the site of metaphysic tibia in OVX rats. However, the positive effects of WE or PR alone on bone and mineral metabolism were diminished when OVX rats were cotreated with WE and PR.
The combination of these two herbs offsets their independent actions on bone and mineral metabolism in vivo. The results of the present study could provide insights to medical professionals to further their understanding of the potential negative impact of herb-herb interactions when a combination of herbal mixtures is used for the management of osteoporosis.
本研究旨在评估女贞子（Fructus Ligustri Lucidi，FLL）和葛根（Puerariae radix，PR）联合治疗对去卵巢（OVX）大鼠骨矿物质代谢的疗效，以期寻找一种替代方案来治疗绝经后骨质疏松症。
将 6 月龄 OVX 大鼠作为绝经后骨质疏松模型，分别将 PR 水提取物（PR）和 FLL 水提取物（WE）添加到商业饮食中，或联合添加到商业饮食中，对 OVX 大鼠进行为期 12 周的治疗。测量骨特性、钙磷吸收和骨生化标志物，以研究 PR 和 WE 对 OVX 大鼠骨和矿物质代谢的潜在相互作用。
长期 PR 治疗并未显著改善骨特性，但极大地改善了 OVX 大鼠继发的甲状旁腺功能亢进。WE 显著提高了肠道钙吸收率，并降低了 OVX 大鼠胫骨近端小梁骨表面积的增大。然而，当 OVX 大鼠同时接受 WE 和 PR 治疗时，WE 或 PR 单独对骨和矿物质代谢的积极作用减弱。
这两种草药的联合使用抵消了它们对体内骨矿物质代谢的独立作用。本研究结果可为医学专业人员提供启示，使其进一步了解当使用草药混合物治疗骨质疏松症时，草药-草药相互作用可能产生的负面影响。
