Fang Ji, Yang Li, Zhang Ronghua, Zhu Xiaofeng, Wang Panpan
Pharmacy College of Jinan University Guangzhou 510632, China.
The First Affiliated Hospital of Jinan University Guangzhou 510630, China.
Int J Clin Exp Pathol. 2015 Feb 1;8(2):1491-502. eCollection 2015.
Ovariectomized Sprague-Dawley (SD) and Wistar rats are widely used as animal models for postmenopausal osteoporosis. This study aimed to investigate differences in the long-term effects of ovariectomy on these two breeds of rats. 6-month-old SD and Wistar rats underwent sham operation (Ssham and Wsham) or bilateral ovariectomy (Sovx and Wovx). Body weight, absolute fat, fifth and sixth lumber vertebrae (L5-6) bone mineral density (BMD), and whole body bone mineral content (BMC) were evaluated from 14 to 68 weeks after operation. Body weight significantly increased compared to shams at every point (except 68 weeks) for Sovx and at 14, 21 and 38 weeks for Wovx (all P < 0.05), the increases were not significantly different between breeds. Absolute fat increased significantly compared to shams at every point (except 14 and 68 weeks) for Sovx and at 30 weeks for Wovx (all P < 0.05), the increases were significantly different between Sovx and Wovx (P = 0.007), but not the shams. L5-6 BMD increased then decreased and was significantly lower than shams at 30, 38 and 50 weeks for Sovx and at 14 weeks for Wovx (all P < 0.05), the initial increases were significantly different between Sovx and Wovx (P = 0.006), but not the shams. BMC was significantly decreased compared to sham groups at every point (except 14 and 68 weeks) for Sovx and at 14, 21, 38, and 50 weeks for Wovx (all P < 0.05), the decreases were not significantly different between breeds. In conclusion, ovariectomy had a greater effect upon absolute fat and L5-6 BMD in SD rats.
去卵巢的斯普拉格-道利（SD）大鼠和Wistar大鼠被广泛用作绝经后骨质疏松症的动物模型。本研究旨在调查去卵巢对这两种品系大鼠的长期影响的差异。6个月大的SD大鼠和Wistar大鼠接受假手术（Ssham和Wsham）或双侧卵巢切除术（Sovx和Wovx）。在术后14至68周评估体重、绝对脂肪量、第五和第六腰椎（L5-6）骨密度（BMD）以及全身骨矿物质含量（BMC）。Sovx组在术后各时间点（68周除外）体重均显著高于假手术组，Wovx组在术后14、21和38周体重显著高于假手术组（均P<0.05），两个品系间体重增加无显著差异。Sovx组在术后各时间点（14和68周除外）绝对脂肪量显著高于假手术组，Wovx组在术后30周绝对脂肪量显著高于假手术组（均P<0.05），Sovx组和Wovx组间绝对脂肪量增加有显著差异（P=0.007），而假手术组间无差异。Sovx组L5-6 BMD先升高后降低，在术后30、38和50周显著低于假手术组，Wovx组在术后14周显著低于假手术组（均P<0.05），Sovx组和Wovx组L5-6 BMD最初升高有显著差异（P=0.006），而假手术组间无差异。Sovx组在术后各时间点（14和68周除外）BMC显著低于假手术组，Wovx组在术后14、21、38和50周BMC显著低于假手术组（均P<0.05），两个品系间BMC降低无显著差异。总之，去卵巢对SD大鼠的绝对脂肪量和L5-6 BMD影响更大。
