使用单克隆抗体NCRC-11检测系统性乳腺癌患者血清中的多态性上皮粘蛋白。
Detection of polymorphic epithelial mucins in the serum of systemic breast cancer patients using the monoclonal antibody, NCRC-11.
作者信息
Price M R, Clarke A J, Robertson J F, O'Sullivan C, Baldwin R W, Blamey R W
机构信息
Cancer Research Campaign Laboratories, University of Nottingham, University Park, UK.
出版信息
Cancer Immunol Immunother. 1990;31(5):269-72. doi: 10.1007/BF01740933.
Serum from patients with systemic breast cancer was found to contain elevated levels of polymorphic epithelial mucin (PEM) as detected using an immunoradiometric assay employing the monoclonal antibody NCRC-11. PEM was partially purified from pooled sera from these patients and the complex, polymorphic, high-molecular-mass (greater than 400 kDa) mucin was identified by sodium dodecylsulphate/polyacrylamide gel electrophoresis, Western blotting and immunostaining with the NCRC-11 antibody. Serial serum samples from 16 patients with metastatic breast cancer were assayed for circulating PEM defined by the monoclonal antibody NCRC-11. The clinical course of disease in these patients was assessed independently as progressive, static or responsive. Increasing NCRC-11 antigen levels correlated with disease progression in 6/7 patients, and decreasing antigen levels correlated with an objective response to treatment in 5/6 patients. Measurement of NCRC-11-defined PEM antigen in patients undergoing therapy for metastatic breast cancer showed an overall accuracy of 75%.
采用使用单克隆抗体NCRC - 11的免疫放射分析检测发现，系统性乳腺癌患者的血清中多形上皮粘蛋白（PEM）水平升高。从这些患者的混合血清中对PEM进行了部分纯化，并通过十二烷基硫酸钠/聚丙烯酰胺凝胶电泳、蛋白质印迹法以及用NCRC - 11抗体进行免疫染色鉴定出这种复杂的、多形的、高分子量（大于400 kDa）的粘蛋白。对16例转移性乳腺癌患者的系列血清样本检测了由单克隆抗体NCRC - 11定义的循环PEM。独立评估这些患者的疾病临床进程为进展期、静止期或反应期。在6/7的患者中，NCRC - 11抗原水平升高与疾病进展相关，在5/6的患者中，抗原水平降低与对治疗的客观反应相关。对转移性乳腺癌治疗患者的NCRC - 11定义的PEM抗原测量显示总体准确率为75%。
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