Price M R, Sekowski M, Yang G Y, Durrant L G, Robins R A, Baldwin R W
Cancer Research Campaign Laboratories, University of Nottingham, UK.
Cancer Immunol Immunother. 1991;33(2):80-4. doi: 10.1007/BF01742533.
A murine anti-(human gastric carcinoma) monoclonal antibody, GL-013 (IgG1), which reacts with a high-molecular-mass glycoprotein from colorectal tumour tissue [Yang and Price (1989) Anticancer Res 9: 1707], was examined for reactivity against a panel of purified and partially purified antigens associated with tumours of the gastrointestinal tract. These included carcinoembryonic antigen (CEA), normal cross-reacting antigen, Y-hapten glycoproteins, and perchloric acid extracts and glycolipid preparations from colorectal tumours. While the GL-013 antibody failed to bind to these antigens, it was found to react strongly with synthetic peptides with sequences based upon that reported for the protein core of a human gastrointestinal mucin [Barnd et al. (1989) Proc Natl Acad Sci USA 86: 7159; Gum et al. (1989) J Biol Chem 264: 6480]. In control tests, a series of other anti-(colorectal tumour) antibodies (IgG1 and IgG3), with broad reactivity towards gastrointestinal carcinomas, as well as an anti-CEA antibody, (IgG1) failed to react with the synthetic peptides. It is concluded that the anti-(gastric carcinoma) monoclonal antibody GL-013 binds to a threonine-rich peptide epitope expressed within the protein core of gastrointestinal mucins.
一种鼠抗（人胃癌）单克隆抗体GL-013（IgG1），它能与结肠肿瘤组织中的一种高分子量糖蛋白发生反应[杨和普赖斯（1989年）《抗癌研究》9：1707]，对一组与胃肠道肿瘤相关的纯化和部分纯化抗原的反应性进行了检测。这些抗原包括癌胚抗原（CEA）、正常交叉反应抗原、Y-半抗原糖蛋白以及来自结肠肿瘤的高氯酸提取物和糖脂制剂。虽然GL-013抗体未能与这些抗原结合，但发现它能与基于报道的人胃肠道粘蛋白蛋白核心序列的合成肽发生强烈反应[巴恩德等人（1989年）《美国国家科学院院刊》86：7159；古姆等人（1989年）《生物化学杂志》264：6480]。在对照试验中，一系列其他抗（结肠肿瘤）抗体（IgG1和IgG3），对胃肠道癌具有广泛反应性，以及一种抗CEA抗体（IgG1）均未能与合成肽发生反应。得出的结论是，抗（胃癌）单克隆抗体GL-013与胃肠道粘蛋白蛋白核心中表达的富含苏氨酸的肽表位结合。
