Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic; Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
Environ Microbiol Rep. 2012 Aug;4(4):459-66. doi: 10.1111/j.1758-2229.2012.00365.x. Epub 2012 Jul 3.
Bacteriocin production was tested in two sets of Escherichia coli strains: one isolated in 1978 from healthy children of rubber tree tappers and Indians in Amazonia, Brazil (n = 35), and the second one isolated in 2004 from healthy adult volunteers in the Moravia region, Czech Republic (n = 53). Although the occurrence of bacteriocin production was rather similar in both groups (54.3% and 43.4% respectively), the spectra of bacteriocin-encoding determinants in both groups were different. Altogether, 12 different bacteriocin-encoding determinants were found among the tested strains. The occurrence of colicin E1- and Y-genes was higher (P = 0.02 and P = 0.009 respectively) while the occurrence of microcin V gene was lower (P = 0.02) among Amazonian strains compared with Moravian strains. The colicin-encoding determinants of colicins Ia, M, Y and E1 were amplified from Amazonian and Moravian producer strains and sequenced, as were chromosomal 16S rRNA, gyrB and tonB genes. While sequence alignments of chromosomal loci revealed no clusters with respect to geographical origin of strains, the colicin-encoding genes were very similar among the strains of each origin but different between the two geographic groups.
一组是 1978 年从巴西亚马逊地区橡胶树种植者和印第安人的健康儿童中分离出来的（n=35），另一组是 2004 年从捷克摩拉维亚地区的健康成年志愿者中分离出来的（n=53）。尽管两组的细菌素产生情况相当相似（分别为 54.3%和 43.4%），但两组的细菌素编码决定簇的谱是不同的。在测试的菌株中总共发现了 12 种不同的细菌素编码决定簇。与摩拉维亚菌株相比，亚马孙菌株中 colicin E1-和 Y-基因的出现频率更高（P=0.02 和 P=0.009），而 microcin V 基因的出现频率更低（P=0.02）。从亚马孙和摩拉维亚的生产菌株中扩增了 colicin Ia、M、Y 和 E1 的编码决定簇，并对其进行了测序，同时还对染色体 16S rRNA、gyrB 和 tonB 基因进行了测序。虽然染色体基因座的序列比对没有显示出与菌株地理起源有关的聚类，但每个起源的菌株中的 colicin 编码基因非常相似，但在两个地理群体之间却有所不同。
