Štaudová Barbora, Micenková Lenka, Bosák Juraj, Hrazdilová Kristýna, Slaninková Eva, Vrba Martin, Ševčíková Alena, Kohoutová Darina, Woznicová Vladana, Bureš Jan, Šmajs David
Department of Biology, Faculty of Medicine, Masaryk University, Kamenice 5, Building A6, 625 00, Brno, Czech Republic.
Department of Infectious Diseases and Microbiology, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Palackého tř. 1/3, 612 42, Brno, Czech Republic.
BMC Microbiol. 2015 Oct 6;15:201. doi: 10.1186/s12866-015-0530-5.
To screen whether E. coli strains encoding type 1 fimbriae, isolated from fecal microflora, produce bacteriocins more often relative to fimA-negative E. coli strains of similar origin.
PCR assays were used to detect presence of genes encoding 30 bacteriocin determinants (23 colicin- and 7 microcin-encoding genes) and 18 virulence determinants in 579 E. coli strains of human and animal origin isolated from hospitals and animal facilities in the Czech and Slovak Republic. E. coli strains were also classified into phylogroups (A, B1, B2 and D).
fimA-negative E. coli strains (defined as those possessing none of the 18 tested virulence determinants) were compared to fimA-positive E. coli strains (possessing fimA as the only detected virulence determinant). Strains with identified bacteriocin genes were more commonly found among fimA-positive E. coli strains (35.6%) compared to fimA-negative E. coli strains (21.9%, p<0.01) and this was true for both colicin and microcin determinants (p=0.02 and p<0.01, respectively). In addition, an increased number of strains encoding colicin E1 were found among fimA-positive E. coli strains (p<0.01).
fimA-positive E. coli strains produced bacteriocins (colicins and microcins) more often compared to fimA-negative strains of similar origin. Since type 1 fimbriae of E. coli have been shown to mediate adhesion to epithelial host cells and help colonize the intestines, bacteriocin synthesis appears to be an additional feature of colonizing E. coli strains.
筛选从粪便微生物群中分离出的编码1型菌毛的大肠杆菌菌株，相对于来源相似的fimA阴性大肠杆菌菌株，是否更频繁地产生细菌素。
采用聚合酶链反应（PCR）检测从捷克和斯洛伐克共和国的医院及动物设施中分离出的579株人和动物源大肠杆菌菌株中30种细菌素决定簇（23种编码大肠杆菌素的基因和7种编码微菌素的基因）及18种毒力决定簇的基因存在情况。大肠杆菌菌株也被分为不同的系统发育群（A、B1、B2和D）。
将fimA阴性大肠杆菌菌株（定义为不具有所检测的18种毒力决定簇中的任何一种）与fimA阳性大肠杆菌菌株（仅检测到fimA作为毒力决定簇）进行比较。与fimA阴性大肠杆菌菌株（21.9%，p<0.01）相比，在fimA阳性大肠杆菌菌株中更常见到具有已鉴定细菌素基因的菌株（35.6%），这对于大肠杆菌素和微菌素决定簇均成立（分别为p=0.02和p<0.01）。此外，在fimA阳性大肠杆菌菌株中发现编码大肠杆菌素E1的菌株数量增加（p<0.01）。
与来源相似的fimA阴性菌株相比，fimA阳性大肠杆菌菌株更频繁地产生细菌素（大肠杆菌素和微菌素）。由于已证明大肠杆菌的1型菌毛可介导与上皮宿主细胞的粘附并有助于在肠道中定殖，细菌素合成似乎是定殖大肠杆菌菌株的另一个特征。
