State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, The Chinese Academy of Sciences, Wuhan, China.
PLoS One. 2013 Jun 7;8(6):e65506. doi: 10.1371/journal.pone.0065506. Print 2013.
The ecological safety of transgenic organisms is an important issue of international public and political concern. The assessment of ecological risks is also crucial for realizing the beneficial industrial application of transgenic organisms. In this study, reproduction of common carp (Cyprinus carpio, CC) in isolated natural aquatic environments was analyzed. Using the method of paternity testing, a comparative analysis was conducted on the structure of an offspring population of "all-fish" growth hormone gene-transgenic common carp (afgh-CC) and of wild CC to evaluate their fertility and juvenile viability. Experimental results showed that in a natural aquatic environment, the ratio of comparative advantage in mating ability of afgh-CC over wild CC was 1∶1, showing nearly identical mating competitiveness. Juvenile viability of afgh-CC was low, and the average daily survival rate was less than 98.00%. After a possible accidental escape or release of transgenic CC into natural aquatic environments they are unable to monopolize resources from eggs of natural CC populations, leading to the extinction of transgenic CC. Transgenic CC are also unlikely to form dominant populations in natural aquatic environments due to their low juvenile viability. Thus, it is expected that the proportion of afgh-CC in the natural environment would remain low or gradually decline, and ultimately disappear.
转基因生物的生态安全性是国际公共和政治关注的重要问题。生态风险评估对于实现转基因生物的有益工业应用也至关重要。本研究分析了隔离自然水生环境中鲤鱼（Cyprinus carpio，CC）的繁殖情况。利用亲子鉴定方法，对“全鱼”生长激素基因转基因鲤鱼（afgh-CC）和野生 CC 的后代种群结构进行了比较分析，以评估它们的繁殖力和幼体活力。实验结果表明，在自然水生环境中，afgh-CC 相对于野生 CC 的交配能力比较优势的比例为 1∶1，表现出几乎相同的交配竞争力。afgh-CC 的幼体活力较低，平均日存活率低于 98.00%。如果转基因 CC 意外逃逸或释放到自然水生环境中，它们无法从天然 CC 种群的卵子中独占资源，从而导致转基因 CC 的灭绝。由于幼体活力低，转基因 CC 也不太可能在自然水生环境中形成优势种群。因此，预计 afgh-CC 在自然环境中的比例将保持较低或逐渐下降，并最终消失。
