Luo Lifei, Huang Rong, Zhang Aidi, Yang Cheng, Chen Liangming, Zhu Denghui, Li Yongming, He Libo, Liao Lanjie, Zhu Zuoyan, Wang Yaping
State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.
University of Chinese Academy of Sciences, Beijing, 100049, China.
Funct Integr Genomics. 2018 Jul;18(4):425-437. doi: 10.1007/s10142-018-0597-9. Epub 2018 Apr 5.
Transgenic Yellow River carp is characterized by rapid growth rate and high feed-conversion efficiency and exhibits a great application prospect. However, there is still a significant separation of growth traits in the transgenic Yellow River carp family; as such, growth-related genotypes must be screened for molecular marker-assisted selection. In this study, 23 growth-related candidate genes containing 48 SNP markers were screened through bulked segregant analysis (BSA) among transgenic Yellow River carp family members showing significant separation of growth traits. Then, two growth-related genes (Nos. 17 and 14 genes) were identified through combined genome-wide association study (GWAS) of candidate genes and validation of the full-sibling family approach. Nos. 17 and 14 genes encode BR serine/threonine-protein kinase 2 (BRSK2) and eukaryotic translation-initiation factor 2-alpha kinase 3 (Eif2ak3), respectively. The average body weight of three subgroups carrying the genotypes 17GG, 17GG + 14CC, and 17GG + 14TT of these two genes increased by 27.96, 38.28, and 33.72%, respectively, compared with the controls. The proportion of individuals with body weight > 500 g in these subgroups increased by 19.22, 26.82, and 30.92%, respectively. The results showed that appropriate genotype carriers can be selected from the progeny population through BSA sequencing combined with simplified GWAS analysis. Hence, basic population for breeding can be constructed and transgenic Yellow River carp strains with stable production performance and uniform phenotypic properties can be bred.
转基因黄河鲤具有生长速度快、饲料转化效率高的特点，具有广阔的应用前景。然而，转基因黄河鲤家系中生长性状仍存在显著分离；因此，必须筛选与生长相关的基因型用于分子标记辅助选择。本研究通过对生长性状有显著分离的转基因黄河鲤家系成员进行混合分组分析法（BSA），筛选出23个与生长相关的候选基因，包含48个单核苷酸多态性（SNP）标记。然后，通过候选基因的全基因组关联研究（GWAS）与全同胞家系方法验证相结合，鉴定出两个与生长相关的基因（第17号和第14号基因）。第17号和第14号基因分别编码BR丝氨酸/苏氨酸蛋白激酶2（BRSK2）和真核翻译起始因子2α激酶3（Eif2ak3）。这两个基因的基因型为17GG、17GG + 14CC和17GG + 14TT的三个亚组的平均体重分别比对照组增加了27.96%、38.28%和33.72%。这些亚组中体重>500 g的个体比例分别增加了19.22%、26.82%和30.92%。结果表明，通过BSA测序结合简化的GWAS分析，可以从后代群体中筛选出合适的基因型携带者。因此，可以构建育种基础群体，培育出生长性能稳定、表型性状一致的转基因黄河鲤品系。
