Takadanohara Hiroshi, Catanzaro Roberto, Chui De Hua, He Fengtian, Yadav Hariom, Ganguli Abhijit, Sakata Yasuhiko, Solimene Umberto, Minelli Emilio, Kobayashi Riyichi, Nagamachi Yoko, Marotta Francesco
Suheiro Chem-Tech Center, Niigata, Japan.
Acta Biomed. 2012 Dec;83(3):208-16.
Increased intestinal permeability has been advocated as one of the likely causes of various pathologies, such as allergies and metabolic or even cardiovascular disturbances. Thus, the aim of the present study was to test a symbiotic preparation containing microbial lysates (KC-1317, Named, Italy) against stress-induced derangement of gut mucosa permeability. Sprague Dawley rats were allocated into control (n=20) and stress (n=20) group. Stress was implemented by 1h of water avoidance stress daily for 10 days. Body weight, food and water intake and passage of stool pellet during stress session were recorded throughout the experiment. On the 11th day, fluorescent iso-thiocyanate dextran solution was injected into small intestinal loops. One hour after the injection, rats were sacrificed. Jejunum and ileum were taken for histopathology. Blood was collected from the abdominal aorta to measure intestinal permeability. In stress group, stool pellets during stress session was significantly higher than control group (p < 0.01). Villus height (p < 0.01), crypt depth (p < 0.01), number of goblet cells in villus (p < 0.01) and crypt (p < 0.05) decreased significantly in jejunum as compared to control. These phenomena were significantly prevented by KC-1317 (p < 0.05). Ileum also showed atrophy but villus height and the number of goblet cells in the villi did not significantly differ. Plasma-concentration of brain-gut peptides (substance P, thyrotropin-releasing hormone, cholecystokinin and motilin) were affected by stress (p < 0.001) and this effect did not change during supplementation with KC-1317. Polymorphonuclear neutrophil counting was significantly higher in stress group as compared to control (p < 0.01) but this phenomenon was abolished in the ileum (p < 0.01) or partly but significantly reduced by KC-1317 supplementation (p < 0.05). Accordingly, intestinal permeability was significantly enhanced in stress group as compared to control (p < 0.01) and prevented by KC-1317 (p < 0.01) in both intestinal segments examined. While confirming that chronic mild stress in rats compromises small intestinal morphology and permeability, we showed that a symbiotic microbial lysate can partly counteract this phenomenon.
肠通透性增加被认为是多种病理状况的可能原因之一，如过敏、代谢紊乱甚至心血管疾病。因此，本研究旨在测试一种含有微生物裂解物的共生制剂（KC - 1317，意大利命名）对抗应激诱导的肠黏膜通透性紊乱的效果。将Sprague Dawley大鼠分为对照组（n = 20）和应激组（n = 20）。通过每天1小时的禁水应激持续10天来施加应激。在整个实验过程中记录应激期间的体重、食物和水摄入量以及粪便颗粒排出情况。在第11天，将异硫氰酸荧光素葡聚糖溶液注入小肠肠袢。注射1小时后，处死大鼠。取空肠和回肠进行组织病理学检查。从腹主动脉采集血液以测量肠通透性。在应激组中，应激期间的粪便颗粒明显多于对照组（p < 0.01）。与对照组相比，空肠的绒毛高度（p < 0.01）、隐窝深度（p < 0.0'）、绒毛杯状细胞数量（p < 0.01）和隐窝杯状细胞数量（p < 0.05）均显著减少。这些现象被KC - 1317显著预防（p < 0.05）。回肠也出现萎缩，但绒毛高度和绒毛杯状细胞数量无显著差异。脑 - 肠肽（P物质、促甲状腺激素释放激素、胆囊收缩素和胃动素）的血浆浓度受应激影响（p < 0.001），并且在补充KC - 1317期间这种影响没有改变。与对照组相比，应激组的多形核中性粒细胞计数显著更高（p < 0.01），但这种现象在回肠中消失（p < 0.01），或者通过补充KC - 1317部分但显著降低（p < 0.05）。因此，与对照组相比，应激组的肠通透性显著增强（p < 0.01），并且在两个检查的肠段中均被KC - 1317预防（p < 0.01）。在证实大鼠慢性轻度应激会损害小肠形态和通透性的同时，我们表明一种共生微生物裂解物可以部分抵消这种现象。
