Key Laboratory for Agro-ecological Processes in Subtropical Region, Research Center for Healthy Breeding of Livestock and Poultry, Institute of Subtropical Agriculture, Chinese Academy of Sciences, 410125 Hunan, China.
Amino Acids. 2012 Aug;43(2):813-21. doi: 10.1007/s00726-011-1137-2. Epub 2011 Nov 9.
The objective of this study was to evaluate the effect of oral supplementation with a combination of arginine and glutamine on the intestinal mucosa and inflammatory cytokines of lipopolysaccharide (LPS)-induced adult rats. Fifty Sprague-Dawley rats (average weight of 185 ± 15 g) were randomly divided into five groups: control group A (CA) and control group B (CB), both orally supplemented with 0.9% saline; group Arg, supplemented with 300 mg/kg day(-1) arginine; group Gln, supplemented with 300 mg/kg day(-1) glutamine; group AG, supplemented with 150 mg/kg day(-1) arginine and 150 mg/kg day(-1) glutamine. The experiment lasted for 2 weeks. Food intake and body weight were measured during the experiment. At 10.00 h of day 15, animals were injected with 4 mg/kg LPS (group CB, Arg, Gln, and AG) or sterile saline (group CA) after supplementation. Then at 14.00 h, all animals were killed and blood and tissue collected. The results showed that compared with group CB, arginine concentration tended to be increased (P > 0.05) in group Arg and AG, while there was no significant difference in glutamine concentration among the groups challenged with LPS. Oral supplementation with arginine or/and glutamine mitigated morphology impairment (lower villus height, P < 0.05) in the jejunum and ileum induced by LPS challenge. LPS administration resulted in a significant increase in TNF-α, IL-1β, IL-6 and IL-10 mRNA abundance. Arginine only significantly decreased TNF-α mRNA abundance in the ileum, while glutamine significantly decreased both TNF-α and IL-10 mRNA in the ileum. A combination of arginine and glutamine significantly decreased TNF-α and IL-1β mRNA abundance in both the jejunum and ileum, while they also significantly decreased anti-inflammatory IL-10 in the ileum. These results revealed that an oral supply of combined arginine and glutamine had more favorable effects on the intestinal mucosa and inflammatory cytokines than a supply of arginine or glutamine alone.
本研究旨在评估口服补充精氨酸和谷氨酰胺混合物对脂多糖（LPS）诱导的成年大鼠肠黏膜和炎性细胞因子的影响。50 只 Sprague-Dawley 大鼠（平均体重 185±15 g）随机分为 5 组：对照组 A（CA）和对照组 B（CB），均口服 0.9%生理盐水；组 Arg，补充 300 mg/kg·天（-1）精氨酸；组 Gln，补充 300 mg/kg·天（-1）谷氨酰胺；组 AG，补充 150 mg/kg·天（-1）精氨酸和 150 mg/kg·天（-1）谷氨酰胺。实验持续 2 周。实验期间测量动物的采食量和体重。第 15 天 10 点，经补充后，除 CA 组外，其余各组动物均腹腔注射 LPS 4mg/kg（CB、Arg、Gln 和 AG 组）或无菌生理盐水。然后在 14 点，处死所有动物并采集血液和组织。结果显示，与 CB 组相比，Arg 组和 AG 组精氨酸浓度有升高趋势（P＞0.05），但 LPS 刺激各组间谷氨酰胺浓度无显著差异。口服补充精氨酸或/和谷氨酰胺可减轻 LPS 刺激引起的空肠和回肠形态损伤（绒毛高度降低，P＜0.05）。LPS 处理导致 TNF-α、IL-1β、IL-6 和 IL-10 mRNA 丰度显著增加。精氨酸仅显著降低回肠 TNF-α mRNA 丰度，而谷氨酰胺显著降低回肠 TNF-α 和 IL-10 mRNA 丰度。精氨酸和谷氨酰胺联合使用可显著降低空肠和回肠 TNF-α 和 IL-1β mRNA 丰度，同时还可显著降低回肠抗炎性 IL-10。这些结果表明，口服补充精氨酸和谷氨酰胺混合物对肠黏膜和炎性细胞因子的影响优于单独补充精氨酸或谷氨酰胺。
