Mycotic and Parasitic Agents and Mycobacteria, Robert Koch-Institut, Nordufer 20, 13353 Berlin, Germany.
J Cyst Fibros. 2013 Dec;12(6):592-8. doi: 10.1016/j.jcf.2013.05.007. Epub 2013 Jun 12.
Scedosporium and Pseudallescheria species are the second most common lung-colonising fungi in cystic fibrosis (CF) patients. For epidemiological reasons it is important to trace sources of infection, routes of transmission and to determine whether these fungi are transient or permanent colonisers of the respiratory tract. Molecular typing methods like multilocus sequence typing (MLST) help provide this data.
Clinical isolates of the P. boydii complex (including S. apiospermum and P. boydii) from CF patients in different regions of Germany were studied using MLST. Five gene loci, ACT, CAL, RPB2, BT2 and SOD2, were analysed.
The S. apiospermum isolates from 34 patients were assigned to 32 sequence types (STs), and the P. boydii isolates from 14 patients to 8 STs. The results revealed that patients can be colonised by individual strains for years.
The MLST scheme developed for S. apiospermum and P. boydii is a highly effective tool for epidemiologic studies worldwide. The MLST data are accessible at http://mlst.mycologylab.org/.
枝孢属和拟青霉属是囊性纤维化（CF）患者肺部第二常见的定植真菌。出于流行病学原因，追踪感染源、传播途径以及确定这些真菌是呼吸道的暂时还是永久性定植者非常重要。多基因座序列分型（MLST）等分子分型方法有助于提供这些数据。
使用 MLST 对来自德国不同地区 CF 患者的拟青霉属复合体（包括枝孢属和拟青霉属）的临床分离株进行研究。分析了 5 个基因座，ACT、CAL、RPB2、BT2 和 SOD2。
34 名患者的枝孢属分离株被分配到 32 个序列型（ST），14 名患者的拟青霉属分离株被分配到 8 个 ST。结果表明，患者可能多年来被个别菌株定植。
为枝孢属和拟青霉属开发的 MLST 方案是全球进行流行病学研究的有效工具。MLST 数据可在 http://mlst.mycologylab.org/ 上获取。
