Guangdong Provincial Public Laboratory of Analysis and Testing Technology, China National Analytical Center Guangzhou, Guangzhou 510070, PR China.
Anal Chim Acta. 2013 Jun 27;785:82-90. doi: 10.1016/j.aca.2013.04.056. Epub 2013 May 9.
Chemical fingerprinting methodology is an approach for quality assessment and control of herbal medicines and related products based on the holistic chemical profile obtained by various analytical techniques. This study demonstrates the first application of paper spray mass spectrometry (PS-MS) as a chemical fingerprinting methodology for tracing the origins, establishing the authenticity, and assessing the overall quality of a famous herbal product, Bansha herbal tea (BHT). A negative ion PS-MS spectrum yielded the best chemical profiling information and was most appropriate for fingerprint analysis of BHT. In addition to the identification of active ingredients, various compounds present in BHT were simultaneously detected without any sample pretreatment and chromatographic separation, providing valuable information for the quality assessment and control of this herbal product. According to the principal component analysis of the PS-MS fingerprints, two sources of commercially available BHT products made by different manufacturers were easily differentiated. Qualified and expired products from the two manufacturers were also successfully distinguished, and the consistency of the quality between the manufacturers was assessed. Our experimental data demonstrated that the PS-MS chemical fingerprinting is a simple, rapid, and robust methodology for pharmaceutical analysis, with promising prospects for quality assessment and control of herbal medicines and related products with high-throughput.
化学指纹图谱方法是一种基于各种分析技术获得的整体化学特征来评估和控制草药及相关产品质量的方法。本研究首次应用纸喷雾质谱（PS-MS）作为一种化学指纹图谱方法，用于追踪起源、确定真实性和评估一种著名草药产品——板沙 herbal tea（BHT）的整体质量。负离子 PS-MS 光谱可提供最佳的化学分析信息，最适合 BHT 的指纹分析。除了鉴定活性成分外，还可以同时检测 BHT 中存在的各种化合物，而无需任何样品预处理和色谱分离，为该草药产品的质量评估和控制提供了有价值的信息。根据 PS-MS 指纹的主成分分析，可以轻松区分由不同制造商生产的两种市售 BHT 产品。还成功区分了来自两个制造商的合格和过期产品，并评估了制造商之间的质量一致性。我们的实验数据表明，PS-MS 化学指纹图谱是一种简单、快速和强大的药物分析方法，具有高通量评估和控制草药及相关产品质量的广阔前景。
