Department of Chemistry, Capital Normal University, Beijing 100048, People's Republic of China.
Nanotechnology. 2013 Jul 12;24(27):275605. doi: 10.1088/0957-4484/24/27/275605. Epub 2013 Jun 14.
Monodispersed truncated octahedral (TOH) Au nanoparticles (NPs) with an average edge-length of about 16 nm were synthesized using poly(diallyldimethylammonium chloride) (PDDA) both as a stabilizing and reducing agent via a one-step reaction. Remarkably, no seeds, surfactants or additional reductant were used in this reaction. In addition, the PDDA molecules on the surface of the TOH AuNPs make them convenient for use in layer-by-layer assembly by electrostatic interactions. Importantly, the TOH AuNPs show a significant surface enhanced Raman scattering (SERS) activity, and can be directly used for building SERS-active substrates and tags. Based on these promising properties, an ultrasensitive SERS-based immunosensing platform was developed. Using human immunoglobulin (h-IgG) as a model target analyte, a detection limit of 36.56 fg ml(-1) was reached.
采用聚二烯丙基二甲基氯化铵（PDDA）作为稳定剂和还原剂，通过一步反应合成了具有约 16nm 平均边长的单分散截断八面体（TOH）金纳米粒子（NPs）。值得注意的是，该反应中未使用种子、表面活性剂或额外的还原剂。此外，TOH AuNPs 表面的 PDDA 分子使其通过静电相互作用方便地用于层层组装。重要的是，TOH AuNPs 表现出显著的表面增强拉曼散射（SERS）活性，可直接用于构建 SERS 活性基底和标记物。基于这些有前景的特性，开发了一种超灵敏的基于 SERS 的免疫传感平台。以人免疫球蛋白（h-IgG）为模型分析物，检测限达到 36.56 fg ml(-1)。
