Beckman Laser Institute and Medical Clinic, 1002 Health Sciences Road, Irvine, California 92617-3010, USA.
J Biomed Opt. 2013 Jun;18(6):66010. doi: 10.1117/1.JBO.18.6.066010.
Frequent monitoring of early-stage burns is necessary for deciding optimal treatment and management. Both superficial and full thickness burns are relatively easy to diagnose based on clinical observation. In between these two extremes are superficial-partial thickness and deep-partial thickness burns. These burns, while visually similar, differ dramatically in terms of clinical treatment and are known to progress in severity over time. The objective of this study was to determine the potential of spatial frequency domain imaging (SFDI) for noninvasively mapping quantitative changes in chromophore and optical properties that may be an indicative of burn wound severity. A controlled protocol of graded burn severity was developed and applied to 17 rats. SFDI data was acquired at multiple near-infrared wavelengths over a course of 3 h. Burn severity was verified using hematoxylin and eosin histology. From this study, we found that changes in water concentration (edema), deoxygenated hemoglobin concentration, and optical scattering (tissue denaturation) to be statistically significant at differentiating superficial partial-thickness burns from deep-partial thickness burns.
频繁监测早期烧伤对于确定最佳治疗和管理方案至关重要。根据临床观察，表浅性和全层性烧伤相对容易诊断。介于这两个极端之间的是表浅-部分厚度烧伤和深-部分厚度烧伤。这些烧伤虽然在外观上相似，但在临床治疗方面却有很大的不同，而且随着时间的推移，其严重程度会逐渐加重。本研究旨在确定空间域成像（SFDI）在无创性绘制可能指示烧伤严重程度的色团和光学性质定量变化方面的潜力。我们制定了一个分级烧伤严重程度的对照方案，并将其应用于 17 只大鼠。在 3 小时的过程中，使用近红外多个波长获取 SFDI 数据。使用苏木精和伊红组织学来验证烧伤的严重程度。从这项研究中，我们发现水浓度（水肿）、去氧血红蛋白浓度和光散射（组织变性）的变化在区分表浅-部分厚度烧伤和深-部分厚度烧伤方面具有统计学意义。
