Department of Laboratory Medicine, Konkuk University School of Medicine, Seoul, Republic of Korea.
Neodin Medical Institute, Seoul, Republic of Korea.
J Med Microbiol. 2013 Sep;62(Pt 9):1301-1306. doi: 10.1099/jmm.0.062950-0. Epub 2013 Jun 13.
We evaluated the performance of the Vitek MS for identification of Gram-positive cocci routinely isolated in clinical microbiology laboratories. With a total of 424 well-characterized isolates, the results of the Vitek MS were compared to those of conventional methods and 16S rRNA gene sequencing. The Vitek MS correctly identified 97.9 % of the isolates tested to species level. The Vitek MS correctly identified the species of 97.2 % of the staphylococci (95.9 % of coagulase-negative staphylococci), 97.8 % of the streptococci, and 100 % of the enterococci. For the identification of Gram-positive cocci isolates, the overall concordance rate between conventional identification and the Vitek MS was 94.5 %. The Vitek MS matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) system can be a reliable and rapid method for the identification of most relevant Gram-positive cocci. In addition, expanding the database of the Vitek MS, especially for coagulase-negative staphylococci, is needed to enhance the performance of the Vitek MS.
我们评估了 Vitek MS 用于鉴定临床微生物学实验室常规分离的革兰阳性球菌的性能。使用总共 424 种特征明确的分离株，将 Vitek MS 的结果与传统方法和 16S rRNA 基因测序进行了比较。Vitek MS 将测试的分离株正确鉴定为种水平的比例为 97.9%。Vitek MS 正确鉴定了葡萄球菌（凝固酶阴性葡萄球菌为 95.9%）、链球菌和肠球菌的 97.2%、97.8%和 100%的分离株。对于革兰阳性球菌分离株的鉴定，传统鉴定与 Vitek MS 之间的总符合率为 94.5%。Vitek MS 基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）系统可以成为鉴定大多数相关革兰阳性球菌的可靠和快速方法。此外，需要扩展 Vitek MS 的数据库，特别是针对凝固酶阴性葡萄球菌，以提高 Vitek MS 的性能。
