Graduate School of Environmental Studies, Tohoku University, Sendai 980-8579, Japan.
Lab Chip. 2013 Aug 7;13(15):3128-35. doi: 10.1039/c3lc50455g.
In this study, we developed a novel method for fabricating microwell arrays constructed from alginate gels, and the alginate gel microwells were used for three-dimensional (3D) cell culture. The alginate gel microwells were fabricated on a patterned ITO electrode using alginate gel electrodeposition. Embryonic stem (ES) cells or hepatocellular carcinoma cells (HepG2) were cultured in the alginate gel microwells containing 3T3 cells. During the culture, embryoid bodies (EBs) or HepG2 spheroids were successfully fabricated in the alginate gel microwells. The oxygen consumption of the EBs indicated that they were successfully cultured. Liver-specific gene expressions of the HepG2 spheroids apparently increased by performing 3D co-culture in the microwell arrays with 3T3 cells. These results show that the alginate gel microwells are a useful 3D culture system.
在这项研究中，我们开发了一种制造由藻酸盐凝胶构成的微井阵列的新方法，并且使用藻酸盐凝胶微井进行了三维（3D）细胞培养。藻酸盐凝胶微井是通过藻酸盐凝胶电沉积在图案化的 ITO 电极上制造的。将胚胎干细胞（ES 细胞）或肝癌细胞（HepG2）培养在含有 3T3 细胞的藻酸盐凝胶微井中。在培养过程中，在藻酸盐凝胶微井中成功地制造了类胚体（EBs）或 HepG2 球体。EBs 的耗氧量表明它们被成功地培养了。通过在具有 3T3 细胞的微井阵列中进行 3D 共培养，HepG2 球体的肝特异性基因表达明显增加。这些结果表明藻酸盐凝胶微井是一种有用的 3D 培养系统。
