Liu Zeyang, Takeuchi Masaru, Nakajima Masahiro, Hasegawa Yasuhisa, Huang Qiang, Fukuda Toshio
Department of Micro-Nano Systems Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.
Department of Micro-Nano Systems Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.
Acta Biomater. 2016 Jun;37:93-100. doi: 10.1016/j.actbio.2016.03.045. Epub 2016 Apr 1.
Cell encapsulation within alginate-poly-l-lysine (PLL) microcapsules has been developed to provide a miniaturized three-dimensional (3D) microenvironment with an aqueous core while promoting development of encapsulated cells into high cell-density structures. In this paper, a novel method for fabricating shape-controlled alginate-PLL microcapsules to construct 3D cell structures based on electrodeposition method is provided. Two-dimensional Ca-alginate cell-laden gel membranes were electrodeposited onto a micro-patterned electrode and further detached from the electrode. The PLL was coated onto the gel structures to form alginate-PLL complex as an outer shell and sodium citric solution was utilized to melt the internal alginate to achieve miniaturized 3D microcapsules (sphere, cuboid, and rod shape). By this proposed method, rat liver cells (RLC-18) formed multi-cellular aggregates with high cell-density after cultivation for 2weeks.
The use of alginate-poly-l-lysine (PLL) microcapsules has shown great potential in fabricating 3D cell structures with high cell density. Despite their success related to their ability to provide a miniaturized microenvironment with an aqueous core, alginate-PLL microcapsules has drawback such as a limited shape-control ability. Because of the mechanism of Ca-induced alginate gel formation, it is still difficult to precisely control the gelation process to produce alginate-PLL microcapsules with specific shape. The present study provides an electrodeposition-based method to generate shape-controlled microcapsules for 3D cell structures. Sphere, cuboid, and rod shaped microcapsules of RLC-18 cells were produced for long-term culture to obtain desired morphologies of cell aggregates.
将细胞封装在海藻酸盐-聚-L-赖氨酸（PLL）微胶囊中，已被开发用于提供一个具有水相核心的小型化三维（3D）微环境，同时促进封装细胞发育成高细胞密度结构。本文提供了一种基于电沉积法制造形状可控的海藻酸盐-PLL微胶囊以构建3D细胞结构的新方法。将二维载有钙藻酸盐细胞的凝胶膜电沉积到微图案化电极上，然后进一步从电极上分离。将PLL涂覆在凝胶结构上以形成作为外壳的海藻酸盐-PLL复合物，并利用柠檬酸钠溶液融化内部海藻酸盐以获得小型化的3D微胶囊（球形、长方体形和棒形）。通过这种方法，大鼠肝细胞（RLC-18）在培养2周后形成了具有高细胞密度的多细胞聚集体。
使用海藻酸盐-聚-L-赖氨酸（PLL）微胶囊在制造具有高细胞密度的3D细胞结构方面已显示出巨大潜力。尽管它们因能够提供具有水相核心的小型化微环境而取得成功，但海藻酸盐-PLL微胶囊存在诸如形状控制能力有限等缺点。由于钙诱导海藻酸盐凝胶形成的机制，仍然难以精确控制凝胶化过程以生产具有特定形状的海藻酸盐-PLL微胶囊。本研究提供了一种基于电沉积的方法来生成用于3D细胞结构的形状可控微胶囊。制备了RLC-18细胞的球形、长方体形和棒形微胶囊用于长期培养，以获得所需的细胞聚集体形态。
