Tyler T A, Genzale J A
Herbert V. Shuster, Inc., Quincy, MA 02171.
J Assoc Off Anal Chem. 1990 May-Jun;73(3):467-9.
A sensitive and precise liquid chromatographic method has been developed for the determination of total niacin in beef, semolina, and cottage cheese. The samples are extracted with Ca(OH)2, cleaned up using C18 Sep-Pak cartridges, and chromatographed on a C18 column using a mobile phase consisting of 23% acetonitrile, 0.10% H3PO4, and 0.10% sodium dodecyl sulfate in water. Niacin is detected using an absorbance detector operating at 254 nm. This chromatographic system produces an unusually sharp peak for niacin, enabling a detection limit of approximately 0.05 mg/100 g. The mean recovery was 99.5% and the coefficients of variation for beef, semolina, and cottage cheese were 3.58, 1.02, and 10.0%, respectively.
已开发出一种灵敏且精确的液相色谱法，用于测定牛肉、粗粒小麦粉和农家干酪中的总烟酸含量。样品用Ca(OH)₂提取，使用C18 Sep-Pak柱进行净化，并在C18柱上进行色谱分析，流动相由23%乙腈、0.10% H₃PO₄和0.10%十二烷基硫酸钠的水溶液组成。使用在254 nm波长下运行的吸光度检测器检测烟酸。该色谱系统产生的烟酸峰异常尖锐，检测限约为0.05 mg/100 g。平均回收率为99.5%，牛肉、粗粒小麦粉和农家干酪的变异系数分别为3.58%、1.02%和10.0%。
