反相液相色谱法测定农产品中的百草枯和敌草快

Reverse-phase liquid chromatographic determination of paraquat and diquat in agricultural products.

作者信息

Nagayama T, Maki T, Kan K, Iida M, Nishima T

机构信息

Tokyo Metropolitan Research Laboratory of Public Health, Division of Food Hygiene, Japan.

出版信息

J Assoc Off Anal Chem. 1987 Nov-Dec;70(6):1008-11.

PMID:3436890

Abstract

A simple, rapid, highly sensitive liquid chromatographic method is described for the quantitative determination of paraquat and diquat residues in agricultural products. Paraquat and diquat are extracted with hot dilute hydrochloric acid and are cleaned up on an Amberlite CG-50 column, followed by reverse-phase liquid chromatography on an NH2 column, with ultraviolet detection at 257 nm (paraquat) and 310 nm (diquat). The minimum detectable concentration of both paraquat and diquat was 0.5 ng per injection, which corresponds to a lower detection limit of approximately 0.02 microgram/g in the original samples. Recoveries of paraquat and diquat added to various samples were greater than 79%, and averaged 91 and 90%, respectively, at the 0.1 and 1.0 microgram/g spiking levels.

摘要

本文描述了一种简单、快速、高灵敏度的液相色谱法，用于定量测定农产品中的百草枯和敌草快残留。百草枯和敌草快用热稀盐酸萃取，在Amberlite CG - 50柱上净化，然后在NH2柱上进行反相液相色谱分析，在257 nm（百草枯）和310 nm（敌草快）处进行紫外检测。百草枯和敌草快的最低检测浓度均为每次进样0.5 ng，这相当于原始样品中约0.02微克/克的较低检测限。添加到各种样品中的百草枯和敌草快的回收率均大于79%，在0.1和1.0微克/克的加标水平下，平均回收率分别为91%和90%。