Laboratory for Experimental Hematology and Stem Cells, Institute for Medical Research, University of Belgrade, Dr. Subotića 4, P.O. Box 102, 11129, Belgrade, Serbia.
Cell Biol Int. 2013 Nov;37(11):1162-70. doi: 10.1002/cbin.10144. Epub 2013 Jul 8.
The aim of this study has been to elucidate how different oxygen levels impact the effects of Interleukin-17 (IL-17) on angiogenic properties of endothelial cells. Two endothelial cell lines, mouse MS-1 and human EA.hy 926, were grown in 20% and 3% O2 and their angiogenic abilities analyzed after IL-17 treatment: proliferation, apoptosis, migration and tubulogenesis. Expression of endothelial nitric oxide synthase (eNOS) and cyclooxygenase-2 (Cox-2) was also measured. Considering EA.hy 926 cell line, hypoxia alone reduced proliferation, survival and migration, but not their ability to form tubules. When cultured at 20% O2 , IL-17 stimulated proliferation, migration and tubulogenesis, whereas a hypoxic environment did not affect their migration and proliferation, but increased their survival and tubulogenic properties. Expression of eNOS and Cox-2 increased by both IL-17 and hypoxia, as well as with their combination. With the MS-1 cell line hypoxia did not affect proliferation, survival, migration and tubule formation. At 20% O2 , IL-17 did not alter their proliferation,but inhibited migration and stimulated tubule formation. At 3% O2 , only the stimulating effect of IL-17 on tubulogenesis was evident. The constitutive expression of eNOS was unaffected by oxygen concentrations or IL-17 supplementation, whereas both IL-17 and hypoxia upregulated Cox-2 expression. Thus the effects of IL-17 on the angiogenic properties of endothelial cells depend on both the cell line used and the oxygen concentration.
本研究旨在阐明不同氧水平如何影响白细胞介素-17（IL-17）对内皮细胞血管生成特性的影响。培养两种内皮细胞系，即小鼠 MS-1 和人 EA.hy 926，在 20%和 3%的 O2 下生长，并在 IL-17 处理后分析其血管生成能力：增殖、凋亡、迁移和管腔形成。还测量了内皮型一氧化氮合酶（eNOS）和环氧化酶-2（Cox-2）的表达。考虑到 EA.hy 926 细胞系，单独缺氧会降低增殖、存活和迁移，但不会影响其形成管腔的能力。当在 20%的 O2 下培养时，IL-17 刺激增殖、迁移和管腔形成，而缺氧环境不会影响它们的迁移和增殖，但会增加它们的存活和管腔形成特性。eNOS 和 Cox-2 的表达均由 IL-17 和缺氧增加，以及它们的组合增加。对于 MS-1 细胞系，缺氧不会影响增殖、存活、迁移和管腔形成。在 20%的 O2 下，IL-17 不会改变其增殖，但抑制迁移并刺激管腔形成。在 3%的 O2 下，只有 IL-17 对管腔形成的刺激作用明显。eNOS 的组成型表达不受氧浓度或 IL-17 补充的影响，而 IL-17 和缺氧均可上调 Cox-2 的表达。因此，IL-17 对内皮细胞血管生成特性的影响取决于所使用的细胞系和氧浓度。
