Weigel Paul H, Padgett-McCue Amy J, Baggenstoss Bruce A
Department of Biochemistry & Molecular Biology, The Oklahoma Center for Medical Glycobiology, Oklahoma City, OK, USA.
Methods Mol Biol. 2013;1022:229-47. doi: 10.1007/978-1-62703-465-4_18.
Detecting and quantifying hyaluronan (HA) made by Class I HA synthase (HAS) and determining the level of activity of these membrane-bound enzymes is critical in studies to understand the normal biology of HA and how changes in HAS activity and HA levels or size are important in inflammatory and other diseases, tumorigenesis, and metastasis. Unlike the products made by the vast majority of glycosyltransferases, HA products are more complicated since they are made as a heterogeneous population of sizes spanning a broad mass range. Three radioactive and nonradioactive assay methods are described that can give the amount of HA made with or without information about the distribution of product sizes.
检测和定量由I类透明质酸合酶（HAS）产生的透明质酸（HA），并确定这些膜结合酶的活性水平，对于理解HA的正常生物学特性，以及HAS活性、HA水平或大小的变化在炎症及其他疾病、肿瘤发生和转移中的重要性的研究至关重要。与绝大多数糖基转移酶产生的产物不同，HA产物更为复杂，因为它们是以跨越广泛质量范围的大小不均一的群体形式产生的。本文描述了三种放射性和非放射性测定方法，这些方法可以给出产生的HA的量，无论是否有关于产物大小分布的信息。
