Liu Bo-Ji, Ma Li-Nan, Su Juan, Jing Wei-Wei, Wei Min-Jie, Sha Xian-Zheng
Department of Radiation Oncology, State Key Laboratory of Oncology in South China, Sun Yat-sen University Cancer Center, SYSUCC, Guangzhou, China.
J Biomed Mater Res A. 2014 Jun;102(6):2055-60. doi: 10.1002/jbm.a.34830. Epub 2013 Jul 15.
Chitosan (CS) is widely used as a scaffold material in tissue engineering. The objective of this study was to test whether porous chitosan membrane (PCSM) coating for Nafion used in implantable sensor reduced fibrous capsule (FC) density and promoted superior vascularization compared with PCSM coating for polytetrafluoroethylene (PTFE). PCSM was fabricated with solvent casting/particulate leaching method using silica gel as porogen and characterized in vitro. Then, PCSM-Nafion and PCSM-PTFE composites were assembled with hydrated PCSM and implanted subcutaneously in rats. The histological analysis was performed in comparison with Nafion and PTFE. Implants were explanted 35, 65, and 100 days after the implantation. Histological assessments indicated that both composites achieved presumed effects of porous coatings on decreasing collagen deposition and promoting angiogenesis. PCSM-PTFE exerted higher collagen deposition by area ratio, both within and outside, compared with that of PCSM-Nafion. Angiogenesis within and outside the PCSM-Nafion both increased over time, but that of the PCSM-PTFE within decreased.
壳聚糖（CS）在组织工程中被广泛用作支架材料。本研究的目的是测试与用于聚四氟乙烯（PTFE）的多孔壳聚糖膜（PCSM）涂层相比，用于植入式传感器的Nafion的PCSM涂层是否能降低纤维囊（FC）密度并促进更好的血管化。采用溶剂浇铸/颗粒沥滤法，以硅胶为致孔剂制备PCSM，并进行体外表征。然后，将PCSM-Nafion和PCSM-PTFE复合材料与水合PCSM组装在一起，皮下植入大鼠体内。与Nafion和PTFE相比进行组织学分析。植入后35、65和100天取出植入物。组织学评估表明，两种复合材料都达到了多孔涂层在减少胶原蛋白沉积和促进血管生成方面的预期效果。与PCSM-Nafion相比，PCSM-PTFE在内部和外部的胶原蛋白沉积面积比更高。PCSM-Nafion内部和外部的血管生成均随时间增加，但PCSM-PTFE内部的血管生成减少。
