Sparks T C, Byford R L, Craig M E, Crosby B L, McKenzie C
Department of Entomology, Louisiana State University Agricultural Center, Baton Rouge 70803.
J Econ Entomol. 1990 Jun;83(3):662-5. doi: 10.1093/jee/83.3.662.
The in vivo metabolism of topically applied 14C-permethrin was determined for adults of pyrethroid-resistant and -susceptible horn flies, Haematobia irritans (L.) at 1, 2, and 6 h after treatment. At 1 and 2 h after treatment, resistant horn flies had significantly higher internal levels of radioactivity (permethrin plus metabolites) compared with adults of the susceptible strain. Analysis of the internal extracts by thin-layer chromatography indicated no differences in the levels of permethrin. However, significantly higher levels of metabolites that co-chromatograph with 3-(2'- or 4'-hydroxyphenoxy)benzyl (1RS) cis/trans-3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropane carboxylate at 1 and 2 h after treatment and (1RS) cis/trans-3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropane carboxylic acid at 1 h after treatment. These results demonstrate that an enhanced penetration and metabolism are present during the early phases of permethrin intoxication. Enhanced metabolism may contribute to the ability of resistant horn flies to survive in the presence of pyrethroids.
在处理后1小时、2小时和6小时，测定了拟除虫菊酯抗性和敏感的角蝇（Haematobia irritans (L.)）成虫经皮局部应用14C-氯菊酯后的体内代谢情况。处理后1小时和2小时，抗性角蝇体内的放射性水平（氯菊酯及其代谢产物）显著高于敏感品系的成虫。通过薄层色谱法分析体内提取物表明，氯菊酯的水平没有差异。然而，在处理后1小时和2小时，与3-(2'-或4'-羟基苯氧基)苄基(1RS)顺式/反式-3-(2,2-二氯乙烯基)-2,2-二甲基环丙烷羧酸酯共色谱的代谢产物水平显著更高，在处理后1小时，与(1RS)顺式/反式-3-(2,2-二氯乙烯基)-2,2-二甲基环丙烷羧酸共色谱的代谢产物水平也显著更高。这些结果表明，在氯菊酯中毒的早期阶段存在增强的渗透和代谢。增强的代谢可能有助于抗性角蝇在拟除虫菊酯存在的情况下存活。
