可可毛色二孢β-葡萄糖苷酶的活性位点。pH值和二恶烷对酶催化反应的影响。
The active site of beta-glucosidase from Botryodiplodia theobromae. Effects of pH and dioxan on enzyme-catalysed reactions.
作者信息
Umezurike G M
出版信息
Biochem J. 1977 Dec 1;167(3):831-3. doi: 10.1042/bj1670831.
Abstract
- The hydrolysis of o-nitrophenyl beta-D-glucopyranoside by the high-molecular-weight beta-glucosidase (beta-D-glucoside glucohydrolase, EC 3.2.1.21) of Botryodiplodia theobromae Pat in the absence or presence of added dioxan was found to be dependent on the ionization of two groups, which appeared to be a carboxyl group and an imidazole group. 2. Dioxan increased the Michaelis constant, Km, but decreased the maximum velocity, V.
摘要
- 发现可可毛色二孢菌（Botryodiplodia theobromae Pat）的高分子量β-葡萄糖苷酶（β-D-葡萄糖苷葡糖水解酶，EC 3.2.1.21）在有无添加二恶烷的情况下，对邻硝基苯基β-D-吡喃葡萄糖苷的水解取决于两个基团的电离，这两个基团似乎是一个羧基和一个咪唑基。2. 二恶烷增加了米氏常数Km，但降低了最大速度V。
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