Miyazaki Dai, Uotani Hitomi, Uotani Ryu, Takenobu Fumie, Inata Koudai, Miyake Atsuko, Inoue Yoshitsugu
Division of Ophthalmology and Visual Science, Faculty of Medicine, Tottori University, 36-1 Nishi-cho, Yonago-shi, Tottori-ken 683-8504, Japan.
Nippon Ganka Gakkai Zasshi. 2013 Apr;117(4):351-6.
To evaluate the efficacy of Gram-Fungiflora Y double staining for corneal scraping samples collected by impression in diagnosing infectious keratitis.
Two hundred and thirteen eyes of 192 patients suspected of having infectious keratitis were retrospectively studied for the sensitivity and specificity of Gram-Fungiflora Y based on final diagnosis.
Of 165 infectious keratitis eyes, 107 had bacterial keratitis, 54 had fungal keratitis, and 15 had Acanthamoeba keratitis. Of these, 147 eyes were positive for one or other of the pathogens by the double staining method (overall sensitivity/specificity was 89.1% (95% confidence interval 83.1%-93.2%)/ 79.1% (64.6%-89.0%)). Sensitivity/specificity of the double staining for each of the pathogens was 88.8% 82.1% for bacterial keratitis, 81.4%/98.1% for fungal keratitis and 80.0%/97.0% for Acanthamoeba keratitis.
The double staining method for impression specimens was effective in diagnosing infectious keratitis with high sensitivity, and was especially useful for the diagnosis of fungal or Acanthamoeba keratitis.
评估革兰-真菌Y双重染色法对通过印片采集的角膜刮片样本诊断感染性角膜炎的有效性。
回顾性研究192例疑似感染性角膜炎患者的213只眼，根据最终诊断评估革兰-真菌Y双重染色法的敏感性和特异性。
165只感染性角膜炎眼中，107只患有细菌性角膜炎，54只患有真菌性角膜炎，15只患有棘阿米巴角膜炎。其中，通过双重染色法，147只眼的一种或多种病原体呈阳性（总体敏感性/特异性为89.1%（95%置信区间83.1%-93.2%）/79.1%（64.6%-89.0%））。每种病原体双重染色的敏感性/特异性分别为：细菌性角膜炎88.8%/82.1%，真菌性角膜炎81.4%/98.1%，棘阿米巴角膜炎80.0%/97.0%。
印片标本双重染色法对感染性角膜炎具有高敏感性的诊断效果，尤其对真菌性或棘阿米巴角膜炎的诊断有用。
