感染性角膜炎中的吖啶橙染色和革兰氏染色

Acridine orange and Gram stains in infectious keratitis.

作者信息

Groden L R, Rodnite J, Brinser J H, Genvert G I

机构信息

Department of Ophthalmology, University of South Florida, College of Medicine, Tampa.

出版信息

Cornea. 1990 Apr;9(2):122-4.

PMID:2328581

Abstract

Two hundred thirty consecutive cases of presumed infectious keratitis were reviewed. Cultures were positive in 49.5% (114 of 230). Acridine orange stain sensitivity (81%) was significantly greater than gram stain sensitivity (65%) (p less than 0.002). Gram stain specificity was 92%, and acridine orange specificity was 89%. In keratitis with low or moderate growth, acridine orange was positive in 73% (61 of 84) as compared with Gram stain 53% (45 of 84), (p less than 0.001). In cases of heavy microbial growth, Gram stain was positive in 97% (29 of 30), acridine orange in 100% (30 of 30). This study demonstrates that both Gram stain and acridine orange stain are excellent in cases of infectious keratitis with heavy microbial growth, but that acridine orange is significantly better than Gram stain in cases with low amounts of organisms.

摘要

回顾了230例连续的疑似感染性角膜炎病例。培养结果阳性的有49.5%（230例中的114例）。吖啶橙染色敏感性（81%）显著高于革兰氏染色敏感性（65%）（p<0.002）。革兰氏染色特异性为92%，吖啶橙特异性为89%。在微生物生长量低或中等的角膜炎中，吖啶橙阳性率为73%（84例中的61例），而革兰氏染色为53%（84例中的45例），（p<0.001）。在微生物大量生长的病例中，革兰氏染色阳性率为97%（30例中的29例），吖啶橙为100%（30例中的30例）。本研究表明，在微生物大量生长的感染性角膜炎病例中，革兰氏染色和吖啶橙染色都很好，但在微生物数量少的病例中，吖啶橙明显优于革兰氏染色。