采用超高效液相色谱法同时测定辣椒粉中游离辣椒红素和辣椒玉红素的含量。

Simultaneous determination of free capsorubin and capsanthin in red pepper powder using u-HPLC.

作者信息

Shim You-Shin, Kim Ki-Jin, Seo Dongwon, Ito Masahito, Nakagawa Hiroaki, Arai Shohei, Ha Jaeho

机构信息

Korea Food Research Institute, Food Analysis Center, 516, Baekhyeon, Bundang, Seongnam, Gyeonggi, 463-746, Republic of Korea.

出版信息

J AOAC Int. 2013 Mar-Apr;96(2):341-5. doi: 10.5740/jaoacint.12-179.

DOI:10.5740/jaoacint.12-179

PMID:23767359

Abstract

A simultaneous ultra-HPLC (u-HPLC) method for the determination of free capsorubin and capsanthin in red pepper powder was validated in terms of its precision, accuracy, and linearity. The u-HPLC separation was performed on an RP C18 column (particle size 2 pm, id 2 mm length 100 mm) and with a photodiode-array detector. The recoveries of capsorubin were greater than 83.8 +/- 1.7%; the LOD and LOQ of the u-HPLC analyses were 0.043 and 0.129 mg/kg, respectively. The intraday and interday precisions for capsorubin were less than 9.01%. The recoveries of capsanthin were greater than 87.7 +/- 1.5%, and the LOD and LOQ were 0.101 and 0.306 mglkg, respectively. The intraday and interday precisions for capsanthin were less than 12.66%. All calibration curves for capsorubin and capsanthin exhibited good linearity (r2 = 0.99) within the tested ranges.

摘要

建立了一种同时测定辣椒粉中游离辣椒红素和辣椒玉红素的超高效液相色谱（u-HPLC）方法，并对其精密度、准确度和线性进行了验证。u-HPLC分离在RP C18柱（粒径2μm，内径2mm，长度100mm）上进行，采用光电二极管阵列检测器。辣椒红素的回收率大于83.8±1.7%；u-HPLC分析的检测限和定量限分别为0.043和0.129mg/kg。辣椒红素的日内和日间精密度均小于9.01%。辣椒玉红素的回收率大于87.7±1.5%，检测限和定量限分别为0.101和0.306mg/kg。辣椒玉红素的日内和日间精密度均小于12.66%。在测试范围内，辣椒红素和辣椒玉红素的所有校准曲线均呈现良好的线性（r2 = 0.99）。