辣椒类胡萝卜素、辣椒红素和辣椒玉红素与活性氧的反应

Reaction of Paprika Carotenoids, Capsanthin and Capsorubin, with Reactive Oxygen Species.

作者信息

Nishino Azusa, Yasui Hiroyuki, Maoka Takashi

机构信息

Institute of Health Sciences, Ezaki Glico Co., Ltd. , 4-6-5 Utajima, Nishiyodogawa-ku, Osaka 555-8502, Japan.

Department of Analytical and Bioinorganic Chemistry, Division of Analytical and Physical Chemistry, Kyoto Pharmaceutical University , 5 Nakauchi-cho, Misasagi, Yamashina-ku, Kyoto 607-8414, Japan.

出版信息

J Agric Food Chem. 2016 Jun 15;64(23):4786-92. doi: 10.1021/acs.jafc.6b01706. Epub 2016 Jun 3.

DOI:10.1021/acs.jafc.6b01706

PMID:27229653

Abstract

The reaction of paprika carotenoids, capsanthin and capsorubin, with reactive oxygen species (ROS), such as superoxide anion radical (·O2(-)), hydroxyl radical (·OH), and singlet oxygen ((1)O2), was analyzed by LC/PDA ESI-MS and ESR spectrometry. Capsanthin formed both the 5,6-epoxide and 5,8-epoxide by reaction with ·O2(-) and ·OH. Furthermore, capsanthin also formed 5,6- and 5,8-endoperoxide on reaction with (1)O2. The same results were obtained in the case of capsanthin diacetate. On the other hand, capsorubin showed higher stability against these ROS. Capsorubin formed 7,8-epoxide on reaction with ·O2(-) and ·OH and 7,8-endoperoxide on reaction with (1)O2.

摘要

通过液相色谱/光电二极管阵列电喷雾质谱联用仪（LC/PDA ESI-MS）和电子自旋共振光谱法（ESR）分析了辣椒中的类胡萝卜素辣椒红素和辣椒玉红素与活性氧（ROS）如超氧阴离子自由基（·O₂⁻）、羟基自由基（·OH）和单线态氧（¹O₂）的反应。辣椒红素与·O₂⁻和·OH反应生成5,6-环氧化物和5,8-环氧化物。此外，辣椒红素与¹O₂反应还生成5,6-和5,8-内过氧化物。辣椒红素二乙酸酯也得到了相同的结果。另一方面，辣椒玉红素对这些活性氧表现出更高的稳定性。辣椒玉红素与·O₂⁻和·OH反应生成7,8-环氧化物，与¹O₂反应生成7,8-内过氧化物。