Kilduff T S, Miller J D, Radeke C M, Sharp F R, Heller H C
Department of Psychiatry, Stanford University School of Medicine, California 94305.
J Neurosci. 1990 Jul;10(7):2463-75. doi: 10.1523/JNEUROSCI.10-07-02463.1990.
Neuronal activity underlying various phases of the mammalian hibernation cycle was investigated using the 14C-2-deoxyglucose (2DG) method. Relative 2DG uptake (R2DGU) values were computed for 96 brain regions across 7 phases of the hibernation cycle: euthermia, 3 body temperature (Tb) intervals during entrance into hibernation, stable deep hibernation, and 2 Tb intervals during arousal from hibernation. Multivariate statistical techniques were employed to identify objectively groups of brain regions whose R2DGU values showed a similar pattern across all phases of hibernation. Factor analysis revealed that most of the variability in R2DGU values for the 96 brain regions across the entire cycle could be accounted for by 3 principal factors. These factors could accurately discriminate the various phases of hibernation on the basis of the R2DGU values alone. Three hypothalamic and 3 cortical regions were identified as possibly mediating the entrance into hibernation because they underwent a change in R2DGU early in entrance into hibernation and loaded strongly on one of the principal factors. Another 4 hypothalamic regions were similarly identified as possibly causally involved in the arousal from hibernation. These results, coupled with characteristic changes in ordinal rank of the 96 brain regions in each phase of hibernation, support the concept that mammalian hibernation is an active, integrated orchestration of neurophysiological events rather than a state entered through a passive process.
利用¹⁴C-2-脱氧葡萄糖（2DG）法研究了哺乳动物冬眠周期各阶段的神经元活动。计算了冬眠周期7个阶段中96个脑区的相对2DG摄取量（R2DGU）值：正常体温期、进入冬眠期间的3个体温（Tb）区间、稳定的深冬眠期以及从冬眠中苏醒期间的2个Tb区间。采用多变量统计技术客观地识别出在冬眠所有阶段其R2DGU值呈现相似模式的脑区组。因子分析表明，整个周期内96个脑区R2DGU值的大部分变异性可由3个主要因子解释。这些因子仅凭R2DGU值就能准确区分冬眠的各个阶段。3个下丘脑区域和3个皮质区域被确定可能介导进入冬眠，因为它们在进入冬眠早期R2DGU发生了变化，并且在其中一个主要因子上有很强的载荷。另外4个下丘脑区域同样被确定可能与从冬眠中苏醒有因果关系。这些结果，再加上96个脑区在冬眠各阶段的顺序排名的特征性变化，支持了这样一种概念，即哺乳动物冬眠是神经生理事件的一种主动的、整合的编排，而不是通过被动过程进入的一种状态。
