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Flavonoids: antioxidants against atherosclerosis.类黄酮：对抗动脉粥样硬化的抗氧化剂。
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2
Flavonoid Glycosides from the Seeds of Litchi chinensis.荔枝种子中的类黄酮糖苷。
J Agric Food Chem. 2011 Feb 23;59(4):1205-9. doi: 10.1021/jf104387y. Epub 2011 Feb 2.
3
Dihydrochalcones: Implication in resistance to oxidative stress and bioactivities against advanced glycation end-products and vasoconstriction.二氢查耳酮：在抵抗氧化应激和生物活性方面对晚期糖基化终产物和血管收缩的影响。
Phytochemistry. 2010 Mar;71(4):443-52. doi: 10.1016/j.phytochem.2009.11.004. Epub 2009 Dec 21.
4
Semi-synthesis of dihydrochalcone derivatives and their in vitro antimicrobial activities.二氢查尔酮衍生物的半合成及其体外抗菌活性。
Planta Med. 2010 Apr;76(6):640-3. doi: 10.1055/s-0029-1240619. Epub 2009 Nov 20.
5
Immunochemical detection of flavonoid glycosides: development, specificity, and application of novel monoclonal antibodies.黄酮苷的免疫化学检测：新型单克隆抗体的研发、特异性及应用
Arch Biochem Biophys. 2008 Aug 15;476(2):124-32. doi: 10.1016/j.abb.2008.01.022. Epub 2008 Feb 5.
6
Anti-atherosclerotic and anti-inflammatory activities of catecholic xanthones and flavonoids isolated from Cudrania tricuspidata.从柘树中分离得到的儿茶酚氧杂蒽酮和黄酮类化合物的抗动脉粥样硬化及抗炎活性
Bioorg Med Chem Lett. 2006 Nov 1;16(21):5580-3. doi: 10.1016/j.bmcl.2006.08.032. Epub 2006 Aug 21.
7
Antimicrobial activity of flavonoids.黄酮类化合物的抗菌活性。
Int J Antimicrob Agents. 2005 Nov;26(5):343-56. doi: 10.1016/j.ijantimicag.2005.09.002.
8
Phlorizin: a review.根皮苷综述
Diabetes Metab Res Rev. 2005 Jan-Feb;21(1):31-8. doi: 10.1002/dmrr.532.
9
Structure-activity relationship studies on chalcone derivatives. the potent inhibition of chemical mediators release.查尔酮衍生物的构效关系研究。对化学介质释放的有效抑制作用。
Bioorg Med Chem. 2003 Jan 2;11(1):105-11. doi: 10.1016/s0968-0896(02)00312-7.
10
The antioxidant activity of phloretin: the disclosure of a new antioxidant pharmacophore in flavonoids.根皮素的抗氧化活性：黄酮类化合物中一种新型抗氧化药效基团的揭示。
Biochem Biophys Res Commun. 2002 Jul 5;295(1):9-13. doi: 10.1016/s0006-291x(02)00618-6.

间接同源竞争酶联免疫吸附法检测一类糖基二氢查耳酮。

Indirect homologous competitive enzyme-linked immunosorbent assay for the detection of a class of glycosylated dihydrochalcones.

机构信息

Department of Entomology and UCD Comprehensive Cancer Center, University of California Davis, Davis, California 95616, United States.

出版信息

J Agric Food Chem. 2013 Jul 17;61(28):6964-70. doi: 10.1021/jf401302y. Epub 2013 Jul 2.

DOI:10.1021/jf401302y
PMID:23767873
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3723731/
Abstract

Hesperetin dihydrochalcone 4'-glucoside, 1, and phloretin 4'-glucoside, 2, belong to a family of dihydrochalcone glycosides that exhibit flavorant properties. In this study was developed a competitive, indirect homologous ELISA for the detection of targets 1 and 2 in fermentation media. Immunogen and coating antigen were prepared by conjugating hapten, 4-(3-oxo-3-(2,6-dihydroxy-4-glucoside phenyl)propyl) benzoic acid, to thyroglobulin and bovine serum albumin, respectively. Antibodies raised in rabbits M6122, M6123, and M6124 and the coating antigen were screened and characterized to determine their optimum concentrations. The optimized ELISA, developed with antibody M6122, gave IC50 values of 27.8 and 21.8 ng/mL for 1 and 2, respectively. Selectivity of the assay was assessed by measuring cross-reactivity of antibody M6122 to related congeners such as aglycones and the 2'-glycosides of hesperetin dihydrochalcone, 5 and phloretin, 6. Antibody M6122 showed very low recognition of 5 and virtually no recognition of the aglycones and 6.

摘要

橙皮素二氢查尔酮 4'-葡萄糖苷 1 和根皮苷 4'-葡萄糖苷 2 属于二氢查尔酮糖苷家族，具有调味特性。本研究开发了一种竞争性间接同源 ELISA ，用于检测发酵培养基中的目标物 1 和 2。免疫原和包被抗原分别通过将半抗原 4-(3-氧代-3-(2,6-二羟基-4-葡萄糖基)苯基)丙基苯甲酸与甲状腺球蛋白和牛血清白蛋白缀合来制备。从兔 M6122、M6123 和 M6124 中制备的抗体和包被抗原进行筛选和表征，以确定其最佳浓度。用抗体 M6122 开发的优化 ELISA 对 1 和 2 的 IC50 值分别为 27.8 和 21.8ng/mL。通过测量抗体 M6122 对相关同系物（如苷元和橙皮素二氢查尔酮的 2'-糖苷 5 和根皮苷 6）的交叉反应性来评估该测定法的选择性。抗体 M6122 对 5 的识别非常低，对苷元和 6 的识别几乎没有。