准确检测复杂甲基化图谱中的甲基化胞嘧啶。

Accurate detection of methylated cytosine in complex methylation landscapes.

机构信息

Centre for Biomarker Research and Development, Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, QLD 4072, Australia.

出版信息

Anal Chem. 2013 Jul 16;85(14):6575-9. doi: 10.1021/ac3031948. Epub 2013 Jul 2.

DOI:10.1021/ac3031948

PMID:23768008

Abstract

Monitoring DNA methylation can be a useful biomarker for disease diagnosis and prognosis. However, monitoring the methylation status of a specific cytosine biomarker is often confounded by heterogeneous peripheral DNA methylation. To address this issue, molecular inversion probes were designed with inosine strategically positioned to complement suspected DNA methylation sites. This enabled the methylation status of a specific cytosine to be accurately measured with a high level of specificity, irrespective of adjacent epigenetic modifications.

摘要

监测 DNA 甲基化可以作为疾病诊断和预后的有用生物标志物。然而，对特定胞嘧啶生物标志物的甲基化状态进行监测常常受到异质性外周 DNA 甲基化的干扰。为了解决这个问题，设计了分子反转探针，其中有肌苷（inosine）被策略性地放置以补充可疑的 DNA 甲基化位点。这使得能够以高特异性准确测量特定胞嘧啶的甲基化状态，而与相邻的表观遗传修饰无关。