Takanashi Kenta, Kato Teru
Graduate School of Bionics, Computer and Media Sciences, Tokyo University of Technology.
Anal Sci. 2014;30(3):371-6. doi: 10.2116/analsci.30.371.
Methylated DNA plays an important role in epigenetic gene regulation, and could therefore serve as a potentially promising biomarker for the diagnosis of cancer and other diseases. Therefore, the development of a simple method for analyzing cytosine methylation in a target gene is required. Here, we report on a new method for the sequence-selective chemical modification of a single cytosine in single-stranded DNA (ssDNA). This method is based on the formation of a DNA three-way junction of the ssDNA and two ssDNA probes, and was successfully applied for a simple polymerase chain reaction (PCR)-based assay for 'pinpoint' methylation analysis.
甲基化DNA在表观遗传基因调控中发挥着重要作用，因此有可能作为一种很有前景的生物标志物用于癌症和其他疾病的诊断。因此，需要开发一种简单的方法来分析目标基因中的胞嘧啶甲基化。在此，我们报告一种对单链DNA（ssDNA）中的单个胞嘧啶进行序列选择性化学修饰的新方法。该方法基于ssDNA与两个ssDNA探针形成DNA三链体结构，并成功应用于基于简单聚合酶链反应（PCR）的“精准”甲基化分析检测。
