BALB/cJ小鼠全身与鼓室内给予类固醇药物后与年龄相关的耳蜗细胞因子基因表达
Age-related cochlear cytokine gene expression in the BALB/cJ mouse with systemic versus intratympanic dosing of steroid drugs.
作者信息
Tokarz Sara A, Pang Jiaqing, Grosz Anna, Kempton J Beth, Trune Dennis R, Pillers De-Ann M
机构信息
Division of Neonatology, Department of Pediatrics, University of Wisconsin-Madison, Meriter Hospital, Madison, WI 53715, USA.
出版信息
Acta Otolaryngol. 2013 Jul;133(7):685-91. doi: 10.3109/00016489.2013.771407.
CONCLUSION
Age-related differences in the expression of inflammatory cytokines in the inner ear may contribute to the development of age-related hearing loss (ARHL).
OBJECTIVES
ARHL is characterized by tissue remodeling, ischemia, ion homeostasis, and inflammation. Steroid therapy is an otoprotective strategy that likely acts by reducing inflammation. We examined age-related changes in cytokine gene expression in the cochlea of the BALB/cJ mouse model of premature ARHL after systemic or intratympanic steroid delivery.
METHODS
'Young' (2.5-3 months) and 'Old' (5-9 months) mice were treated with dexamethasone or fludrocortisone administered either orally or intratympanically. Cytokine gene expression in cochlear RNA was analyzed using prefabricated cDNA arrays. Old groups were compared to Young groups to identify age-related changes.
RESULTS
Down-regulation of a cytokine associated with bone remodeling (SPP1) was observed in the untreated Old group. Numerous genes were up- or down-regulated by more than twofold by steroid treatment, including proinflammatory interleukins (IL-16) and anti-inflammatory cytokines.
结论
内耳中炎症细胞因子表达的年龄相关性差异可能促成年龄相关性听力损失（ARHL）的发生。
目的
ARHL的特征为组织重塑、缺血、离子稳态及炎症。类固醇疗法是一种耳保护策略，可能通过减轻炎症起作用。我们研究了在全身或鼓室内给予类固醇后，过早发生ARHL的BALB/cJ小鼠模型耳蜗中细胞因子基因表达的年龄相关性变化。
方法
“年轻”（2.5 - 3个月）和“年老”（5 - 9个月）小鼠接受口服或鼓室内给予地塞米松或氟氢可的松治疗。使用预制的cDNA阵列分析耳蜗RNA中的细胞因子基因表达。将老年组与年轻组进行比较，以确定年龄相关性变化。
结果
在未治疗的老年组中观察到与骨重塑相关的细胞因子（SPP1）下调。类固醇治疗使众多基因上调或下调超过两倍，包括促炎白细胞介素（IL - 16）和抗炎细胞因子。
Zotero 插件