Department of Microbiology and Molecular Biology, Brigham Young University, Provo, UT 84602, USA.
Asian Pac J Trop Med. 2013 Jul;6(7):552-5. doi: 10.1016/S1995-7645(13)60095-4.
To determine if passive gravitational sedimentation of blood samples, followed by buffy coat thin smear preparation could increase the sensitivity of malaria diagnosis when compared to conventional thin smear preparation without the additional cost of centrifuges or molecular diagnostics.
Blood samples were collected from 205 patients. Each patient sample was analyzed using all three methods of sample preparation.
Buffy coat analysis of centrifuged blood samples greatly increased the sensitivity of malaria diagnosis when compared to standard thin smear techniques. Sensitivity between mechanically centrifuged samples and gravitationally sedimented samples showed equal improvement in sensitivity when compared to standard thin smear preparation.
Passive gravitational sedimentation of red blood cells followed by buffy coat analysis dramatically improves the sensitivity of malaria diagnosis without the additional costs associated with centrifugation.
确定与不使用离心机或分子诊断的常规薄涂片制备相比，血液样本的被动重力沉降，随后进行白细胞层薄涂片制备是否可以提高疟疾诊断的敏感性。
采集了 205 名患者的血液样本。每个患者样本均使用三种样品制备方法进行分析。
与标准薄涂片技术相比，离心血液样本的白细胞层分析大大提高了疟疾诊断的敏感性。与标准薄涂片制备相比，机械离心样本和重力沉降样本之间的敏感性均显示出敏感性的同等提高。
红细胞的被动重力沉降，随后进行白细胞层分析，无需与离心相关的额外费用，即可显著提高疟疾诊断的敏感性。
