Molecular and Cell Biology Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, 18KM Khazar Blvd, Khazar Sq. Sari, Iran.
Asian Pac J Trop Med. 2013 Jul;6(7):556-60. doi: 10.1016/S1995-7645(13)60096-6.
To determine the patterns of distribution of HCV genotypes among high risk population in north of Iran.
A cross-sectional study was conducted on 135 HCV RNA-positive high risk individuals including thalassemia, hemophilia, patients under hemodialysis and intravenous drug addicts. HCV genotypes were determined based on amplification with type-specific primers methods.
Among the 187 anti-HCV positive samples, only 135 (72.2%) gave HCV-RNA positvity. Over all, the most identified HCV type was genotype 3a (51.1%) followed by 1a (27.4%), 1b (8.2%). Sixteen (11.9%) out of 135 HCV RNA-positive participants have infected with more than one genotype or subtypes as follow; 1a/1b in 11 (8.2%), 2/3a in 3 (2.2%), and 1a/1b/3a in 2 (1.5%). Stratification of participants revealed that HCV subtype 3a was more prominent in thalassemia, hemophilia and HD patients but 1a and 1b were frequent in intravenous drug addicts.
This study is the first report on HCV genotypes among Iranian subjects with different exposure categories resided in Mazandaran, where genotype 3a was found to be the most frequent genotype in thalassemia, hemophilia, and hemodialysis patients but not in IDAs. Since the addiction age is decreasing in Iran and a lot of addicts are IDAs, it might change the subtype pattern of HCV in general population.
确定伊朗北部高危人群中 HCV 基因型的分布模式。
对包括地中海贫血、血友病、血液透析患者和静脉药物滥用者在内的 135 名 HCV RNA 阳性高危个体进行了一项横断面研究。采用基于特异性引物扩增的方法确定 HCV 基因型。
在 187 份抗 HCV 阳性样本中，只有 135 份（72.2%）HCV-RNA 阳性。总体而言，最常见的 HCV 类型是 3a 基因型（51.1%），其次是 1a 基因型（27.4%）、1b 基因型（8.2%）。135 名 HCV RNA 阳性参与者中有 16 名（11.9%）感染了一种以上基因型或亚型，包括 1a/1b（8.2%）、2/3a（2.2%）和 1a/1b/3a（1.5%）。分层分析显示，HCV 亚型 3a 在地中海贫血、血友病和血液透析患者中更为突出，而 1a 和 1b 则在静脉药物滥用者中更为常见。
本研究是关于不同暴露类别伊朗人群中 HCV 基因型的首次报告，其中 3a 基因型在接受输血、血友病和血液透析的患者中最为常见，但在静脉药物滥用者中并非如此。由于伊朗的吸毒年龄正在下降，而且大量吸毒者是静脉药物滥用者，这可能会改变 HCV 在一般人群中的亚型模式。
