College of Pharmacy, Xi'an Jiaotong University, Xi'an 710061, PR China.
Toxicol Appl Pharmacol. 2013 Oct 1;272(1):21-9. doi: 10.1016/j.taap.2013.05.035. Epub 2013 Jun 13.
Excessive alcohol consumption can lead to gastric ulcer and the present work was aimed to examine the protective effect of tetrahydrocoptisine (THC) in the model of ethanol-induced gastric ulcer in mice. Fasted mice treated with ethanol 75% (0.5ml/100g) were pre-treated with THC (10 or 20mg/kg, ip), cimetidine (100mg/kg, ip) or saline in different experimental sets for a period of 3days, and animals were euthanized 4h after ethanol ingestion. Gross and microscopic lesions, immunological and biochemical parameters were taken into consideration. The results showed that ethanol induced gastric damage, improving nitric oxide (NO) level, increased pro-inflammatory cytokine (TNF-α and IL-6) levels and myeloperoxidase (MPO) activity, as well as the expression of nuclear factor-κB (NF-κB) in the ethanol group. Pretreatment of THC at doses of 10 and 20mg/kg bodyweight significantly attenuated the gastric lesions as compared to the ethanol group. These results suggest that the gastroprotective activity of THC is attributed to reducing NO production and adjusting the pro-inflammatory cytokine, inhibited neutrophil accumulation and NF-κB expression.
过量饮酒可导致胃溃疡，本研究旨在探讨四氢黄连碱（THC）对乙醇诱导的小鼠胃溃疡模型的保护作用。禁食后，给予乙醇 75%（0.5ml/100g）的小鼠用 THC（10 或 20mg/kg，ip）、西咪替丁（100mg/kg，ip）或生理盐水在不同的实验条件下预处理 3 天，在摄入乙醇后 4 小时处死动物。考虑到大体和微观病变、免疫和生化参数。结果表明，乙醇诱导胃损伤，提高一氧化氮（NO）水平，增加促炎细胞因子（TNF-α和 IL-6）水平和髓过氧化物酶（MPO）活性，以及核因子-κB（NF-κB）在乙醇组中的表达。与乙醇组相比，THC 以 10 和 20mg/kg 体重的剂量预处理可显著减轻胃损伤。这些结果表明，THC 的胃保护活性归因于减少 NO 生成和调节促炎细胞因子，抑制中性粒细胞聚集和 NF-κB 表达。
