Jiangsu University of Science and Technology, Zhenjiang, Jiangsu 212003, China.
Gene. 2013 Sep 15;527(1):161-6. doi: 10.1016/j.gene.2013.05.049. Epub 2013 Jun 11.
The "Ming" lethal egg mutant (l-em) is a vitelline membrane mutant in silkworm, Bombyx mori. The eggs laid by the l-em mutant lose water, ultimately causing death within an hour. Previous studies have shown that the deletion of BmEP80 is responsible for the l-em mutation in silkworm, B. mori. In the current study, digital gene expression (DGE) was performed to investigate the difference of gene expression in ovaries between wild type and l-em mutant on the sixth day of the pupal stage to obtain a global view of gene expression profiles using the ovaries of three l-em mutants and three wild types. The results showed a total of 3,463,495 and 3,607,936 clean tags in the wild type and the l-em mutant libraries, respectively. Compared with those of wild type, 239 differentially expressed genes were detected in the l-em mutant, wherein 181 genes are up-regulated and 58 genes are down-regulated in the mutant strain. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis results showed that no pathway was significantly enriched and three pathways are tightly related to protein synthesis among the five leading pathways. Moreover, the expression profiles of eight important differentially expressed genes related to oogenesis changed. These results provide a comprehensive gene expression analysis of oogenesis and vitellogenesis in B. mori which facilitates understanding of both the specific molecular mechanism of the 1-em mutant and Lepidopteran oogenesis in general.
“明”致死卵突变体（l-em）是家蚕的卵壳突变体。l-em 突变体产下的卵会失去水分，最终在一小时内死亡。先前的研究表明，BmEP80 的缺失是导致家蚕 l-em 突变的原因。在本研究中，采用数字基因表达（DGE）技术，对蛹期第 6 天野生型和 l-em 突变型家蚕卵巢中的基因表达差异进行了研究，以获得卵巢中基因表达谱的全局视图，共使用了 3 个 l-em 突变体和 3 个野生型。结果显示，野生型和 l-em 突变体文库中分别有 3463495 和 3607936 个清洁标签。与野生型相比，在 l-em 突变体中检测到 239 个差异表达基因，其中 181 个基因上调，58 个基因下调。京都基因与基因组百科全书（KEGG）通路分析结果表明，在 5 个主要通路中，没有显著富集的通路，有 3 个通路与蛋白质合成密切相关。此外，8 个与卵母细胞发生相关的重要差异表达基因的表达谱发生了变化。这些结果为家蚕卵母细胞发生和卵黄发生提供了全面的基因表达分析，有助于理解 1-em 突变体的特定分子机制以及鳞翅目昆虫卵母细胞发生的一般机制。
