Gynecology Department, Changning Maternity and Infant Health Hospital, Shanghai, China.
FEBS Lett. 2013 Aug 19;587(16):2523-9. doi: 10.1016/j.febslet.2013.05.065. Epub 2013 Jun 12.
MicroRNAs negatively regulate target gene expression at the post-transcriptional level during carcinogenesis. Recent advances revealed that the expression levels of several miRNAs are up- or down-regulated in endometrial carcinoma (EC). Here we identify dysregulated miRNAs in EC and we elucidate the essential role of let-7a. The expression of 86 miRNAs in EC was found to be different from adjacent normal endometrial tissues. Moreover, miR-let-7 members are down-regulated in EC and let-7 miRNAs are highly associated with endometrial cancer. A functional investigation revealed that let-7a suppressed proliferation of HeLa cells by targeting Aurora-B. Let-7a also antagonizes Aurora-B functions in promoting carcinoma cell proliferation by down-regulating Aurora-B protein level. Let-7a could be applied for gene therapy against endometrial carcinogenesis.
微小 RNA 在前致癌过程中在转录后水平负调控靶基因的表达。最近的研究进展表明，几种微小 RNA 的表达水平在子宫内膜癌 (EC) 中上调或下调。在这里，我们鉴定了 EC 中失调的微小 RNA，并阐明了 let-7a 的重要作用。发现 EC 中的 86 种微小 RNA 的表达与相邻的正常子宫内膜组织不同。此外，miR-let-7 成员在 EC 中下调，并且 let-7 miRNAs 与子宫内膜癌高度相关。功能研究表明，let-7a 通过靶向 Aurora-B 抑制 HeLa 细胞的增殖。let-7a 还通过下调 Aurora-B 蛋白水平拮抗 Aurora-B 促进癌细胞增殖的功能。let-7a 可用于针对子宫内膜癌发生的基因治疗。
