细胞质簇蛋白表达与胰腺神经内分泌肿瘤的大小和病理分期相关。
Cytoplasmic Clusterin expression correlates with pancreatic neuroendocrine tumor size and pathological stage.
机构信息
Department of Anatomic Pathology, Moffitt Cancer Center and University of South Florida, College of Medicine, Tampa, FL 33612, USA.
出版信息
Pancreas. 2013 Aug;42(6):967-70. doi: 10.1097/MPA.0b013e318293734b.
OBJECTIVES
Cytoplasmic clusterin (Clusterin), a ubiquitous multifunctional secretory sulfated glycoprotein, plays a role in apoptosis and is reportedly overexpressed in a variety of tumors. The role of Clusterin in pancreatic neuroendocrine tumors (PNETs) has not been investigated. In this study, Clusterin expression was evaluated in a subset of PNETs, and the results were correlated with the clinical-pathological features of the tumors.
METHODS
Fifty-nine surgical cases were used to evaluate the immunohistochemical expression of Clusterin in PNETs. Using the avidin-biotin complex method, tissue sections from each case were stained with a rabbit anticlusterin antibody (Abcam, Cambridge, Mass). The immunohistochemical reactions were qualitatively and semiquantitatively evaluated by 2 pathologists.
RESULTS
Strong Clusterin reactivity was identified in 36 (61%) of 59 PNETs. In 23 (39%) of 59 cases, the Clusterin score was 3 or less. Clusterin expression scores significantly associated with tumor size (P = 0.03) and with tumor stage (P = 0.02). The immunohistochemical score index did not correlate with tumor grade (P = 0.15).
CONCLUSIONS
We report the expression of Clusterin in PNETs. The correlation of Clusterin with tumor size and stage suggests involvement of this molecule in pancreatic neuroendocrine tumor progression. Clusterin may represent a new target of therapy for PNETs.
目的
细胞质聚集素（Clusterin）是一种普遍存在的多功能分泌性硫酸化糖蛋白，在细胞凋亡中发挥作用，据报道在多种肿瘤中过度表达。Clusterin 在胰腺神经内分泌肿瘤（PNETs）中的作用尚未被研究。在本研究中，评估了 Clusterin 在 PNETs 中的表达，并将结果与肿瘤的临床病理特征相关联。
方法
使用 59 例手术病例评估 Clusterin 在 PNETs 中的免疫组织化学表达。使用亲和素-生物素复合物法，用兔抗 Clusterin 抗体（Abcam，马萨诸塞州剑桥）对每个病例的组织切片进行染色。两位病理学家对免疫组织化学反应进行定性和半定量评估。
结果
在 59 例 PNETs 中，有 36 例（61%）存在强烈的 Clusterin 反应性。在 59 例中的 23 例（39%）中，Clusterin 评分≤3。Clusterin 表达评分与肿瘤大小（P=0.03）和肿瘤分期（P=0.02）显著相关。免疫组化评分指数与肿瘤分级无相关性（P=0.15）。
结论
我们报告了 Clusterin 在 PNETs 中的表达。Clusterin 与肿瘤大小和分期的相关性提示该分子参与了胰腺神经内分泌肿瘤的进展。Clusterin 可能成为 PNETs 的新治疗靶点。
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