Edmunds Marie-Claire, Wigmore Stephen, Kluth David
Department of Surgery, Royal Infirmary of Edinburgh.
J Vis Exp. 2013 Jun 8(76):50473. doi: 10.3791/50473.
Free tissue transfer is the gold standard of reconstructive surgery to repair complex defects not amenable to local options or those requiring composite tissue. Ischemia reperfusion injury (IRI) is a known cause of partial free flap failure and has no effective treatment. Establishing a laboratory model of this injury can prove costly both financially as larger mammals are conventionally used and in the expertise required by the technical difficulty of these procedures typically requires employing an experienced microsurgeon. This publication and video demonstrate the effective use of a model of IRI in rats which does not require microsurgical expertise. This procedure is an in situ model of a transverse abdominis myocutaneous (TRAM) flap where atraumatic clamps are utilized to reproduce the ischemia-reperfusion injury associated with this surgery. A laser Doppler Imaging (LDI) scanner is employed to assess flap perfusion and the image processing software, Image J to assess percentage area skin survival as a primary outcome measure of injury.
游离组织移植是修复复杂缺损（无法采用局部修复方法或需要复合组织修复的缺损）的重建手术的金标准。缺血再灌注损伤（IRI）是游离皮瓣部分坏死的已知原因，且尚无有效治疗方法。建立这种损伤的实验室模型在经济上可能成本高昂，因为传统上使用较大的哺乳动物，而且这些手术的技术难度通常需要有经验的显微外科医生，这也增加了专业技术要求。本出版物及视频展示了一种无需显微外科专业技术的大鼠IRI模型的有效应用。该手术是腹直肌肌皮瓣（TRAM瓣）的原位模型，使用无创夹来重现与此手术相关的缺血再灌注损伤。使用激光多普勒成像（LDI）扫描仪评估皮瓣灌注情况，并使用图像处理软件Image J评估皮肤存活面积百分比，将其作为损伤的主要结局指标。
