Department of Pathology and Key Laboratory of Xinjiang Endemic and Ethnic Diseases Ministry of Education, Shihezi University School of Medicine, Shihezi, Xinjiang 832002, China.
Viral Immunol. 2013 Jun;26(3):180-4. doi: 10.1089/vim.2012.0085.
HLA-G is a class I HLA that has gained much attention due to its multiple functions in the immune system. More important, some studies found HLA-G may be detrimental in tumors and viral infections, and the detection of HLA-G expression might serve as a clinical marker in the prediction of clinical outcomes for certain types of carcinoma. We assessed the association between the development of Kazakh esophageal squamous cell carcinoma (ESCC) harboring high-risk HPV infection and the expression of HLA-G. The expression of HLA-G was detected by S-P immunohistochemical staining in 60 cases of Kazakh ESCC tissues and 40 cases of Kazakh tumor adjacent normal tissues. HPV16 infection in ESCC was detected by genotype-specific polymerase chain reaction. HPV16 infection rate in Kazakh ESCC was 35.0%, significantly higher than that of the infection rate of the adjacent normal tissues 15% (p<0.05, OR=3.051; 95% CI: 1.103-8.438). The expression of HLA-G in Kazakh ESCC was 75.0% (45/60), significantly higher than that of tumor adjacent normal tissues (17.5%; p<0.05); expression of HLA-G was slightly higher in HPV16-positive than HPV16-negative ESCC, but the difference was not statistically significant (p>0.05). The positive expression rate of HLA-G was closely related to depth of invasion and clinical stage (p<0.05 for all), but was not related to age, sex, tumor location, histologic grade, and nodal status (p>0.05 for all). Overexpression of HLA-G was a characteristic feature of Kazakh ESCC; HLA-G may be involved in Kazakh ESCC carcinogenesis and HPV infection.
HLA-G 是一种 I 类 HLA，因其在免疫系统中的多种功能而备受关注。更重要的是，一些研究发现 HLA-G 可能在肿瘤和病毒感染中有害，HLA-G 表达的检测可能作为某些类型的癌临床结果预测的临床标志物。我们评估了哈萨克族食管鳞状细胞癌（ESCC）中高危 HPV 感染的发展与 HLA-G 表达之间的关系。采用 S-P 免疫组化染色法检测 60 例哈萨克族 ESCC 组织和 40 例哈萨克族肿瘤旁正常组织中 HLA-G 的表达。采用基因特异性聚合酶链反应检测 ESCC 中的 HPV16 感染。哈萨克族 ESCC 中 HPV16 感染率为 35.0%，显著高于相邻正常组织的 15%（p<0.05，OR=3.051；95%CI：1.103-8.438）。哈萨克族 ESCC 中 HLA-G 的表达率为 75.0%（45/60），显著高于肿瘤旁正常组织（17.5%；p<0.05）；HPV16 阳性 ESCC 中 HLA-G 的表达略高于 HPV16 阴性 ESCC，但差异无统计学意义（p>0.05）。HLA-G 的阳性表达率与浸润深度和临床分期密切相关（均 p<0.05），但与年龄、性别、肿瘤部位、组织学分级和淋巴结状态无关（均 p>0.05）。HLA-G 的过度表达是哈萨克族 ESCC 的特征；HLA-G 可能参与哈萨克族 ESCC 的癌变和 HPV 感染。
