Zheng Jianyong, Xu Chunsheng, Chu Dake, Zhang Xiaoying, Li Jipeng, Ji Gang, Hong Liu, Feng Quanxin, Li Xiaohua, Wu Guosheng, Du Jianjun, Zhao Qingchuan
State Key Laboratory of Cancer Biology, Department of Digestive Surgery, Xijing Hospital of Digestive Diseases, The Fourth Military Medical University, Xi'an, Shaanxi Province 710032, China.
State Key Laboratory of Cancer Biology, Department of Digestive Surgery, Xijing Hospital of Digestive Diseases, The Fourth Military Medical University, Xi'an, Shaanxi Province 710032, China.
Immunol Lett. 2014 Sep;161(1):13-9. doi: 10.1016/j.imlet.2014.04.007. Epub 2014 Apr 21.
Human leukocyte antigen G (HLA-G) is a non-classical HLA class I molecule thought to play a key role in maternal-fetal tolerance and cancer immune evasion. This study aimed to investigate the HLA-G expression in lesion sections and plasma sHLA-G levels of primary esophageal squamous cell carcinoma (ESCC) patients and its clinical significance in diagnosis and prognosis of ESCC. 60 ESCC patients and 28 healthy controls were recruited, and the positive expression of HLA-G in ESCC lesions and adjacent normal tissues were 70% (42/60) and 8.6% (5/60) (P<0.05), respectively, while no expression was found in normal controls. HLA-G1 and HLA-G5 were determined to be dominating isoforms measured by RT-PCR. There was a significant difference in plasma sHLA-G levels between patients with ESCC (15.04 U/ml, range 4.33-250.00 U/ml) and healthy controls (6.81 U/ml, range 0-29.27 U/ml) (P<0.01). The plasma IL-10 level was higher in ESCC patients than the controls (23.86 pg/ml vs. 12.81 pg/ml, P<0.01). HLA-G expression in lesion tissues was correlated with cancer cell differentiation (P=0.033), lymph node metastasis (P=0.035) of ESCC. However, no obvious correlations were demonstrated between the plasma sHLA-G levels and the clinicopathological parameters. There was a significant correlation between sHLA-G and IL-10 expression (r=0.353, P=0.006) in patients with Esophageal squamous cell carcinoma. HLA-G positive expression showed poorer prognosis of ESCC. HLA-G positive expression might serve as a potential marker in the diagnosis or prediction of ESCC.
人类白细胞抗原G（HLA-G）是一种非经典的I类HLA分子，被认为在母胎耐受和癌症免疫逃逸中起关键作用。本研究旨在探讨原发性食管鳞状细胞癌（ESCC）患者病变组织中HLA-G的表达及血浆可溶性HLA-G（sHLA-G）水平，及其在ESCC诊断和预后中的临床意义。招募了60例ESCC患者和28例健康对照，ESCC病变组织和癌旁正常组织中HLA-G的阳性表达率分别为70%（42/60）和8.6%（5/60）（P<0.05），而正常对照中未发现表达。通过逆转录聚合酶链反应（RT-PCR）测定，HLA-G1和HLA-G5为主要异构体。ESCC患者血浆sHLA-G水平（15.04 U/ml，范围4.33 - 250.00 U/ml）与健康对照（6.81 U/ml，范围0 - 29.27 U/ml）之间存在显著差异（P<0.01）。ESCC患者血浆白细胞介素-10（IL-10）水平高于对照组（23.86 pg/ml对12.81 pg/ml，P<0.01）。病变组织中HLA-G表达与ESCC的癌细胞分化（P=0.033）、淋巴结转移（P=0.035）相关。然而，血浆sHLA-G水平与临床病理参数之间未显示明显相关性。食管鳞状细胞癌患者中sHLA-G与IL-10表达之间存在显著相关性（r=0.353，P=0.006）。HLA-G阳性表达显示ESCC预后较差。HLA-G阳性表达可能作为ESCC诊断或预测的潜在标志物。
