Department of Obstetrics and Perinatology, Medical University of Lublin, Poland.
J Reprod Immunol. 2013 Sep;99(1-2):33-8. doi: 10.1016/j.jri.2013.04.004. Epub 2013 Jun 14.
The aim of our study was to estimate the expression of B7-H1 and B7-H4 molecules on myeloid and plasmacytoid dendritic cells (DCs) in the peripheral blood of patients with pre-eclampsia, normal pregnant women and healthy non-pregnant women. Thirty-three patients with pre-eclampsia, 26 normal pregnant women, and 12 healthy non-pregnant women were included in the study. Dendritic cells were isolated from peripheral blood, stained with monoclonal antibodies against blood dendritic cell antigens and B7-H1 and B7-H4 molecules and estimated using flow cytometry. The expression of B7-H1 and B7-H4 molecules was significantly higher on CD1c(+) myeloid and CD303(+) plasmacytoid DCs in the first trimester of pregnancy than in the luteal phase of the ovarian cycle (CD1c(+)B7-H1(+): 19.19±10.55% vs. 11.99±6.79%; p<0.05; CD1c(+)B7-H4(+): 12.01±9.15% vs. 3.98±1.97%, p<0.001; CD303(+)B7-H1(+): 4.15±2.38% vs. 1.70±0.87%, p<0.05; CD303(+)B7-H4(+): 5.44±2.93% vs. 2.33±1.54%, p<0.01). Moreover, the expression of the B7-H1 molecule on CD1c(+) DCs in the second trimester of normal pregnancy was significantly higher than in the first trimester, but in the third trimester they decreased compared with the second trimester (II vs. I trimester: 32.23±11.30% vs. 19.19±10.55%, p<0.01; III vs. II trimester: 32.23±11.30% vs. 22.39±8.19%, p<0.01). The expression of B7-H1 molecule on CD1c(+) myeloid and CD303(+) plasmacytoid DCs was significantly lower in pre-eclampsia than in healthy third-trimester pregnant women (CD1c(+)B7-H1(+): 13.78±6.26% vs. 22.39±8.19%, p<0.05; CD303(+)B7-H1(+): 3.66±2.46% vs. 8.65±3.15%, p<0.01). Higher expressions of B7-H1 and B7-H4 molecules on CD1c(+) myeloid and CD303(+) plasmacytoid DCs in the first trimester of pregnancy suggest the role they play in the immunomodulation during early pregnancy.
我们的研究目的是评估子痫前期、正常妊娠和健康未孕妇女外周血中髓样和浆细胞样树突状细胞（DCs）上 B7-H1 和 B7-H4 分子的表达。研究纳入了 33 例子痫前期患者、26 例正常妊娠妇女和 12 例健康未孕妇女。从外周血中分离树突状细胞，用针对血液树突状细胞抗原和 B7-H1 和 B7-H4 分子的单克隆抗体染色，并通过流式细胞术进行评估。在妊娠早期，CD1c(+)髓样和 CD303(+)浆细胞样 DC 上 B7-H1 和 B7-H4 分子的表达明显高于卵巢周期黄体期（CD1c(+)B7-H1(+)：19.19±10.55%比 11.99±6.79%；p<0.05；CD1c(+)B7-H4(+)：12.01±9.15%比 3.98±1.97%，p<0.001；CD303(+)B7-H1(+)：4.15±2.38%比 1.70±0.87%，p<0.05；CD303(+)B7-H4(+)：5.44±2.93%比 2.33±1.54%，p<0.01）。此外，正常妊娠中期 CD1c(+)DC 上 B7-H1 分子的表达明显高于妊娠早期，但在妊娠晚期与中期相比有所下降（II 期与 I 期相比：32.23±11.30%比 19.19±10.55%，p<0.01；III 期与 II 期相比：32.23±11.30%比 22.39±8.19%，p<0.01）。子痫前期患者 CD1c(+)髓样和 CD303(+)浆细胞样 DC 上 B7-H1 分子的表达明显低于健康妊娠晚期妇女（CD1c(+)B7-H1(+)：13.78±6.26%比 22.39±8.19%，p<0.05；CD303(+)B7-H1(+)：3.66±2.46%比 8.65±3.15%，p<0.01）。妊娠早期 CD1c(+)髓样和 CD303(+)浆细胞样 DC 上 B7-H1 和 B7-H4 分子的高表达表明它们在妊娠早期免疫调节中发挥作用。
