Lai Xiao-Juan, Chen Hai-Min, Yang Rui, Yan Xiao-Jun
Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University, Ningbo 315211, China.
Yi Chuan. 2013 Jun;35(6):735-44. doi: 10.3724/sp.j.1005.2013.00735.
Algae possess complex and diverse biology and evolutionary history. They play an important role in the ecosystem. A mass of unique genes and biological processes also make them attractive. The application of high-throughput sequencing approaches to algal research has greatly contributed to the development of algal genomics and transcriptomics, as well as enriched the gene information of algae. In this article, we summarize the advances of algal genomics and transcriptomics, describe and compare the characteristics of different algae genomes, and introduce the application of expression sequence tags (ESTs), microarray and RNA sequencing technique to algal transcriptomics research. Furthermore, the advances of algal gene expression and small RNA are also reviewed in detail. At last, we discuss the challenges and future development of this area.
藻类具有复杂多样的生物学特性和进化历史。它们在生态系统中发挥着重要作用。大量独特的基因和生物学过程也使它们具有吸引力。高通量测序方法在藻类研究中的应用极大地推动了藻类基因组学和转录组学的发展，同时也丰富了藻类的基因信息。在本文中，我们总结了藻类基因组学和转录组学的进展，描述并比较了不同藻类基因组的特征，介绍了表达序列标签（ESTs）、微阵列和RNA测序技术在藻类转录组学研究中的应用。此外，还详细综述了藻类基因表达和小RNA的进展。最后，我们讨论了该领域面临的挑战和未来发展。
