• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

H-DNA形成的DNA序列和金属离子特异性。

DNA-sequence and metal-ion specificity of the formation of *H-DNA.

作者信息

Bernués J, Beltrán R, Casasnovas J M, Azorín F

机构信息

Grupo de Química Macromolecular, Centro de Investigación y Desarrollo CSIC, ETSEIB, Barcelona, Spain.

出版信息

Nucleic Acids Res. 1990 Jul 25;18(14):4067-73. doi: 10.1093/nar/18.14.4067.

DOI:10.1093/nar/18.14.4067
PMID:2377452
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC331161/
Abstract

The homopyrimidine-homopurine sequence d(CT/GA)22 undergoes, in the presence of zinc ions, transition to an altered DNA conformation (*H-DNA) which is neither H-DNA nor B-DNA. *H-DNA is characterized by a peculiar chemical reactivity pattern in which most of the polypyrimidine strand is hyperreactive to osmium tetroxide and the central part of the polypurine strand is sensitive to diethylpyrocarbonate. Formation of *H-DNA is specific of metal-ion. *H-DNA is detected in the presence of Zn++, Cd++ and Mn++. The efficiency on promoting the transition is in the order of Zn++ greater than Cd++ much greater than Mn++. Formation of *H-DNA is also specific of nucleotide sequence. From all the different homopolymeric sequences tested only the d(CT/GA)22 sequence showed the zinc-induced transition to *H-DNA. These results suggest that stabilization of *H-DNA involves the formation of a specific complex between the metal-ion and the nucleotide sequence. The biological relevance of these results is discussed in view of the important role that zinc ions play on many nucleic acids processes.

摘要

同嘧啶-同嘌呤序列d(CT/GA)22在锌离子存在下会转变为一种既非H-DNA也非B-DNA的改变的DNA构象（*H-DNA）。*H-DNA的特征在于一种特殊的化学反应模式，其中大部分多嘧啶链对四氧化锇高度反应，而多嘌呤链的中心部分对焦碳酸二乙酯敏感。H-DNA的形成具有金属离子特异性。在Zn++、Cd++和Mn++存在下可检测到H-DNA。促进转变的效率顺序为Zn++大于Cd++远大于Mn++。H-DNA的形成也具有核苷酸序列特异性。在所有测试的不同同聚序列中，只有d(CT/GA)22序列显示出锌诱导的向H-DNA的转变。这些结果表明，*H-DNA的稳定涉及金属离子与核苷酸序列之间形成特定复合物。鉴于锌离子在许多核酸过程中发挥的重要作用，讨论了这些结果的生物学相关性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ff2/331161/21cdb0f358a3/nar00198-0031-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ff2/331161/ca613b89ee5f/nar00198-0028-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ff2/331161/73276663a405/nar00198-0029-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ff2/331161/4e2762268cf1/nar00198-0030-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ff2/331161/21cdb0f358a3/nar00198-0031-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ff2/331161/ca613b89ee5f/nar00198-0028-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ff2/331161/73276663a405/nar00198-0029-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ff2/331161/4e2762268cf1/nar00198-0030-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ff2/331161/21cdb0f358a3/nar00198-0031-a.jpg

相似文献

1
DNA-sequence and metal-ion specificity of the formation of *H-DNA.H-DNA形成的DNA序列和金属离子特异性。
Nucleic Acids Res. 1990 Jul 25;18(14):4067-73. doi: 10.1093/nar/18.14.4067.
2
Structural polymorphism of homopurine--homopyrimidine sequences: the secondary DNA structure adopted by a d(GA.CT)22 sequence in the presence of zinc ions.同嘌呤-同嘧啶序列的结构多态性：锌离子存在时d(GA.CT)22序列所采用的二级DNA结构
EMBO J. 1989 Jul;8(7):2087-94. doi: 10.1002/j.1460-2075.1989.tb03617.x.
3
Structural polymorphism of homopurine-homopyrimidine sequences at neutral pH.中性pH条件下嘌呤-嘧啶同聚物序列的结构多态性
J Mol Biol. 1993 Jun 20;231(4):1090-101. doi: 10.1006/jmbi.1993.1354.
4
The interaction of zinc(II) ions with antiparallel-stranded d(GA)n DNA homoduplexes.锌（II）离子与反平行链d（GA）n DNA同型双链体的相互作用。
J Biomol Struct Dyn. 1998 Oct;16(2):243-51. doi: 10.1080/07391102.1998.10508243.
5
DIVALENT METAL ION ACTIVATION OF BETA-METHYLASPARTASE.β-甲基天冬氨酸酶的二价金属离子激活作用
J Biol Chem. 1964 May;239:1635-9.
6
Characterization of the zinc-induced structural transition to *H-DNA at a d(GA.CT)22 sequence.锌诱导d(GA.CT)22序列向H-DNA结构转变的表征。
J Mol Biol. 1993 Apr 5;230(3):966-78. doi: 10.1006/jmbi.1993.1213.
7
Ion antagonisms in microorganisms; interference of normal magnesium metabolism by nickel, cobalt, cadmium, zinc, and manganese.微生物中的离子拮抗作用；镍、钴、镉、锌和锰对正常镁代谢的干扰。
J Bacteriol. 1950 Oct;60(4):401-13. doi: 10.1128/jb.60.4.401-413.1950.
8
The effect of zinc on the secondary structure of d(GA.TC)n DNA sequences of different length: a model for the formation *H-DNA.锌对不同长度的d(GA.TC)n DNA序列二级结构的影响：一种H-DNA形成模型
Nucleic Acids Res. 1993 Jun 11;21(11):2557-62. doi: 10.1093/nar/21.11.2557.
9
In vitro selection of deoxyribozymes active with Cd(2+) ions resulting in variants of DNAzyme 8-17.体外筛选对Cd(2+)离子有活性的脱氧核酶，从而得到DNAzyme 8-17的变体。
Dalton Trans. 2015 May 7;44(17):8138-49. doi: 10.1039/c5dt00187k.
10
[COMPARATIVE TOXICITY OF VARIOUS METAL IONS AND THEIR ETHYLENEDIAMINE-TETRAACETIC ACID CHELATE].[各种金属离子及其乙二胺四乙酸螯合物的比较毒性]
Pathol Biol. 1963 Aug-Sep;11:853-65.

引用本文的文献

1
Anti-gene oligonucleotides targeting Friedreich's ataxia expanded GAA⋅TTC repeats increase Frataxin expression.靶向弗里德赖希共济失调扩展的GAA⋅TTC重复序列的反基因寡核苷酸可增加frataxin表达。
Mol Ther Nucleic Acids. 2025 Apr 17;36(2):102541. doi: 10.1016/j.omtn.2025.102541. eCollection 2025 Jun 10.
2
Triplex H-DNA structure: the long and winding road from the discovery to its role in human disease.三链体H-DNA结构：从发现到其在人类疾病中作用的漫长曲折之路。
NAR Mol Med. 2024 Dec 5;1(4):ugae024. doi: 10.1093/narmme/ugae024. eCollection 2024 Oct.
3
Mechanism of copper mediated triple helix formation at neutral pH in Drosophila satellite repeats.

本文引用的文献

1
Helical periodicity of DNA, Poly(dA) . poly(dT) and poly(dA-dT). poly(dA-dT) in solution.溶液中DNA、聚（dA）·聚（dT）和聚（dA-dT）·聚（dA-dT）的螺旋周期性。
Eur J Biochem. 1981 Aug;118(2):215-22. doi: 10.1111/j.1432-1033.1981.tb06389.x.
2
Facile transition of poly[d(TG) x d(CA)] into a left-handed helix in physiological conditions.在生理条件下，聚[d(TG)x d(CA)]能轻松转变为左手螺旋。
Nature. 1983 Apr 14;302(5909):632-4. doi: 10.1038/302632a0.
3
Zinc Z-DNA.锌与Z型DNA
果蝇卫星重复序列中铜在中性pH条件下介导三链螺旋形成的机制。
Biophys J. 2007 Apr 1;92(7):2498-506. doi: 10.1529/biophysj.106.099788. Epub 2007 Jan 5.
4
Length-dependent structure formation in Friedreich ataxia (GAA)n*(TTC)n repeats at neutral pH.在中性pH条件下，弗里德赖希共济失调（GAA）n*(TTC)n重复序列中与长度相关的结构形成
Nucleic Acids Res. 2004 Feb 20;32(3):1224-31. doi: 10.1093/nar/gkh274. Print 2004.
5
Parallel intramolecular DNA triple helix with G and T bases in the third strand stabilized by Zn(2+) ions.由锌离子稳定的、第三条链中含有G和T碱基的平行分子内DNA三链螺旋。
Nucleic Acids Res. 2000 Sep 15;28(18):3511-6. doi: 10.1093/nar/28.18.3511.
6
Inhibition of transcription factor IIIA-DNA interactions by xenobiotic metal ions.异源生物金属离子对转录因子IIIA与DNA相互作用的抑制作用。
Nucleic Acids Res. 1996 Mar 1;24(5):924-30. doi: 10.1093/nar/24.5.924.
7
Conformational transitions of alternating purine-pyrimidine DNAs in perchlorate ethanol solutions.高氯酸盐乙醇溶液中交替嘌呤-嘧啶DNA的构象转变
Biophys J. 1995 Nov;69(5):2033-43. doi: 10.1016/S0006-3495(95)80073-1.
8
Cation and sequence effects on stability of intermolecular pyrimidine-purine-purine triplex.阳离子和序列对分子间嘧啶-嘌呤-嘌呤三链体稳定性的影响。
Nucleic Acids Res. 1993 Feb 11;21(3):585-91. doi: 10.1093/nar/21.3.585.
9
Protonated pyrimidine-purine-purine triplex.质子化嘧啶-嘌呤-嘌呤三链体
Nucleic Acids Res. 1993 Jan 11;21(1):105-11. doi: 10.1093/nar/21.1.105.
10
The effect of zinc on the secondary structure of d(GA.TC)n DNA sequences of different length: a model for the formation *H-DNA.锌对不同长度的d(GA.TC)n DNA序列二级结构的影响：一种H-DNA形成模型
Nucleic Acids Res. 1993 Jun 11;21(11):2557-62. doi: 10.1093/nar/21.11.2557.
Nucleic Acids Res. 1984 Apr 25;12(8):3643-8. doi: 10.1093/nar/12.8.3643.
4
The chemistry and biology of left-handed Z-DNA.左手螺旋Z-DNA的化学与生物学
Annu Rev Biochem. 1984;53:791-846. doi: 10.1146/annurev.bi.53.070184.004043.
5
The supercoil-stabilised cruciform of ColE1 is hyper-reactive to osmium tetroxide.ColE1的超螺旋稳定十字形结构对四氧化锇具有高反应性。
EMBO J. 1984 May;3(5):1187-92. doi: 10.1002/j.1460-2075.1984.tb01949.x.
6
Homocopolymer sequences in the spacer of a sea urchin histone gene repeat are sensitive to S1 nuclease.海胆组蛋白基因重复序列间隔区中的同聚物序列对S1核酸酶敏感。
Nature. 1982 Feb 25;295(5851):714-6. doi: 10.1038/295714a0.
7
Sequencing end-labeled DNA with base-specific chemical cleavages.通过碱基特异性化学切割对末端标记的DNA进行测序。
Methods Enzymol. 1980;65(1):499-560. doi: 10.1016/s0076-6879(80)65059-9.
8
Human U1 RNA genes contain an unusually sensitive nuclease S1 cleavage site within the conserved 3' flanking region.人类U1 RNA基因在保守的3'侧翼区域内含有一个异常敏感的核酸酶S1切割位点。
Proc Natl Acad Sci U S A. 1984 Dec;81(23):7288-92. doi: 10.1073/pnas.81.23.7288.
9
Chemical probes of DNA conformation: detection of Z-DNA at nucleotide resolution.DNA构象的化学探针：核苷酸分辨率下Z-DNA的检测
Cell. 1985 Oct;42(3):713-24. doi: 10.1016/0092-8674(85)90268-5.
10
Recognition of the structural distortions at the junctions between B and Z segments in negatively supercoiled DNA by osmium tetroxide.四氧化锇对负超螺旋DNA中B段和Z段交界处结构畸变的识别。
J Biomol Struct Dyn. 1985 Dec;3(3):467-78. doi: 10.1080/07391102.1985.10508435.