具有包含包装信号的修饰长末端重复序列的逆转录病毒载体的高效复制、整合和包装。
Efficient replication, integration, and packaging of retroviral vectors with modified long terminal repeats containing the packaging signal.
作者信息
Joshi S, Van Brunschot A, Robson I, Bernstein A
机构信息
Department of Microbiology, University of Toronto, Ontario, Canada.
出版信息
Nucleic Acids Res. 1990 Jul 25;18(14):4223-6. doi: 10.1093/nar/18.14.4223.
Abstract
Retroviral vectors were modified to contain packaging (psi) signals of varying lengths (nucleotides 211-355, 211-565, or 211-1039 of MoMuLV RNA) between the U3-r and U5 sequences of their 5' long terminal repeat (LTR). For the vector MoTN-PR3, containing the full length 211-1039 nucleotide-long psi signal within the 5' LTR, replication, integration, and packaging were almost as efficient as for the original unmodified vector. This result confirmed that the 211-1039 nucleotide-long sequence from the MoMuLV RNA is sufficient and necessary to allow efficient packaging of RNAs. In addition, an important site was revealed where insertion of foreign DNA sequences of up to 829 nucleotides can be made within the 5' LTR, between U3-r and U5 sequences, without affecting viral replication, integration, or packaging.
摘要
逆转录病毒载体经过修饰，使其5'长末端重复序列（LTR）的U3-r和U5序列之间包含不同长度（莫洛尼鼠白血病病毒（MoMuLV）RNA的核苷酸211 - 355、211 - 565或211 - 1039）的包装（ψ）信号。对于载体MoTN-PR3，其5' LTR内含有全长211 - 1039个核苷酸长的ψ信号，其复制、整合和包装效率几乎与原始未修饰载体相同。这一结果证实，来自MoMuLV RNA的211 - 1039个核苷酸长的序列对于RNA的有效包装是充分且必要的。此外，还发现了一个重要位点，在5' LTR的U3-r和U5序列之间可插入长达829个核苷酸的外源DNA序列，而不影响病毒的复制、整合或包装。
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