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2
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The U3 region of Moloney murine leukemia virus contains position-independent cis-acting sequences involved in the nuclear export of full-length viral transcripts.莫洛尼鼠白血病病毒的U3区域包含参与全长病毒转录本核输出的位置独立顺式作用序列。
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Resistance to human immunodeficiency virus type 1 (HIV-1) infection in human CD4+ lymphocyte-derived cell lines conferred by using retroviral vectors expressing an HIV-1 RNA-specific ribozyme.利用表达HIV-1 RNA特异性核酶的逆转录病毒载体赋予人CD4 +淋巴细胞衍生细胞系对1型人类免疫缺陷病毒（HIV-1）感染的抗性。
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Inhibition of human immunodeficiency virus type 1 multiplication by antisense and sense RNA expression.通过反义RNA和正义RNA表达抑制人类免疫缺陷病毒1型的增殖。
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Construction of a retrovirus packaging mutant and its use to produce helper-free defective retrovirus.逆转录病毒包装突变体的构建及其用于产生无辅助病毒的缺陷型逆转录病毒。
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Deletion mutants of Moloney murine leukemia virus which lack glycosylated gag protein are replication competent.缺乏糖基化gag蛋白的莫洛尼鼠白血病病毒缺失突变体具有复制能力。
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Retrovirus transduction: generation of infectious retroviruses expressing dominant and selectable genes is associated with in vivo recombination and deletion events.逆转录病毒转导：表达显性和可选择基因的感染性逆转录病毒的产生与体内重组和缺失事件相关。
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Form and function of retroviral proviruses.逆转录病毒前病毒的形式与功能。
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Optimal computer folding of large RNA sequences using thermodynamics and auxiliary information.利用热力学和辅助信息对大型RNA序列进行最优计算机折叠
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A new technique for the assay of infectivity of human adenovirus 5 DNA.一种检测人腺病毒5型DNA感染性的新技术。
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Varying the position of a retrovirus packaging sequence results in the encapsidation of both unspliced and spliced RNAs.改变逆转录病毒包装序列的位置会导致未剪接和剪接后的RNA都被包装。
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Replication-competent Moloney murine leukemia virus carrying a bacterial suppressor tRNA gene: selective cloning of proviral and flanking host sequences.携带细菌抑制性tRNA基因的具有复制能力的莫洛尼鼠白血病病毒：前病毒及侧翼宿主序列的选择性克隆
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Evidence that the packaging signal of Moloney murine leukemia virus extends into the gag region.莫洛尼鼠白血病病毒包装信号延伸至gag区域的证据。
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具有包含包装信号的修饰长末端重复序列的逆转录病毒载体的高效复制、整合和包装。

Efficient replication, integration, and packaging of retroviral vectors with modified long terminal repeats containing the packaging signal.

作者信息

Joshi S, Van Brunschot A, Robson I, Bernstein A

机构信息

Department of Microbiology, University of Toronto, Ontario, Canada.

出版信息

Nucleic Acids Res. 1990 Jul 25;18(14):4223-6. doi: 10.1093/nar/18.14.4223.

DOI:10.1093/nar/18.14.4223
PMID:2377461
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC331182/
Abstract

Retroviral vectors were modified to contain packaging (psi) signals of varying lengths (nucleotides 211-355, 211-565, or 211-1039 of MoMuLV RNA) between the U3-r and U5 sequences of their 5' long terminal repeat (LTR). For the vector MoTN-PR3, containing the full length 211-1039 nucleotide-long psi signal within the 5' LTR, replication, integration, and packaging were almost as efficient as for the original unmodified vector. This result confirmed that the 211-1039 nucleotide-long sequence from the MoMuLV RNA is sufficient and necessary to allow efficient packaging of RNAs. In addition, an important site was revealed where insertion of foreign DNA sequences of up to 829 nucleotides can be made within the 5' LTR, between U3-r and U5 sequences, without affecting viral replication, integration, or packaging.

摘要

逆转录病毒载体经过修饰，使其5'长末端重复序列（LTR）的U3-r和U5序列之间包含不同长度（莫洛尼鼠白血病病毒（MoMuLV）RNA的核苷酸211 - 355、211 - 565或211 - 1039）的包装（ψ）信号。对于载体MoTN-PR3，其5' LTR内含有全长211 - 1039个核苷酸长的ψ信号，其复制、整合和包装效率几乎与原始未修饰载体相同。这一结果证实，来自MoMuLV RNA的211 - 1039个核苷酸长的序列对于RNA的有效包装是充分且必要的。此外，还发现了一个重要位点，在5' LTR的U3-r和U5序列之间可插入长达829个核苷酸的外源DNA序列，而不影响病毒的复制、整合或包装。