Key Laboratory of Groundwater Resources and Environment, Ministry of Education, Jilin University, Changchun 130021, China.
J Hazard Mater. 2013 Sep 15;260:323-9. doi: 10.1016/j.jhazmat.2013.05.033. Epub 2013 May 28.
A cold-tolerant microbial consortium, which can use nitrobenzene (NB) and aniline (AN) as sole carbon, nitrogen and energy sources, was isolated from an NB and AN contaminated site. Pilot 454 pyrosequencing analysis of the consortium showed that it was mainly made up of Pseudomonas spp. (98%). At 10 °C, the consortium degraded the mixture of 50mg/L NB and 50mg/L AN at a similar rate as those achieved at 20 °C and 30 °C. The biodegradation by-products with different initial NB and AN concentrations at 10 °C were analyzed. Azobenzene, azoxybenzene and acetanilide were observed in NB and AN mixtures degradation. These by-products are generated by the reaction between different intermediates resulting from the NB and AN degradation as well as the parent compounds. To the best of our knowledge, this is the first report confirming the by-products of NB and AN mixture biodegradation by a cold-tolerant microbial consortium.
从一个受硝基苯 (NB) 和苯胺 (AN) 污染的地点中分离出了一种耐寒微生物群落，该群落可以将 NB 和 AN 用作唯一的碳、氮和能源。对该群落的 454 焦磷酸测序分析表明，它主要由假单胞菌属组成（98%）。在 10°C 时，该群落以与 20°C 和 30°C 相似的速率降解 50mg/L NB 和 50mg/L AN 的混合物。分析了在 10°C 时具有不同初始 NB 和 AN 浓度的生物降解副产物。在 NB 和 AN 混合物的降解过程中观察到偶氮苯、氧化偶氮苯和乙酰苯胺。这些副产物是由 NB 和 AN 降解过程中产生的不同中间产物以及母体化合物之间的反应生成的。据我们所知，这是首次报道耐寒微生物群落对 NB 和 AN 混合物生物降解的副产物的确认。
